Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.

Journal of Biomaterials Science. Polymer Edition
Ji LiYongquan Gu

Abstract

Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels. However, some challenges still exist. We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties. In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times. In decellularization phase II, we systematically compared five combined decellularization protocols based on the results of phase I to identify the optimal method. These protocols tested cell removal, ECM preservation, mechanical properties, and residual cytotoxicity. We further immobilized heparin to optimal decellularized scaffolds and determined its anticoagulant activity and mechanical properties. The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better. In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to ...Continue Reading

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Citations

Feb 16, 2021·Frontiers in Cardiovascular Medicine·Bruna B J LealPatricia Pranke

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Methods Mentioned

BETA
surgical bypass
coronary bypass
vascular bypass
PCAs
Assay
vascular surgical bypass

Software Mentioned

Graph Pad Prism

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