PMID: 8615018Apr 1, 1996Paper

Characterization of a highly conserved baculovirus structural protein that is specific for occlusion-derived virions

Virology
D A TheilmannN E Crook

Abstract

A highly conserved baculovirus late gene called odvp-6e was shown to be a structural protein that is specific for occlusion-derived virus (ODV) envelopes. The complete sequence of this gene is presented for both Orgyia pseudotsugata nuclear polyhedrosis virus (OpMNPV) and Cydia pomonella granulosis virus (CpGV). The predicted sizes of the OpMNPV and CpGV ODVP-6E are 40, 241, and 38,655 respectively. The OpMNPV odvp-6e gene was transcriptionally mapped and was shown to initiate from a consensus late gene motif, TTAAG, and is expressed from 18-120 hr postinfection. Polyclonal antiserum was generated against a bacterial fusion protein and used to analyze the cellular steady-state levels of ODVP-6E and to determine if this protein was a component of either budded virus (BV) or ODV. Western blots showed that ODVP-6E is a component of the ODV but not BV. This was confirmed by immunoelectron microscopy of ODV from Autographa californica NPV (AcMNPV) which localized ODVP-6E to the ODV envelope. The sequences of the odvp-6e gene from the baculoviruses Choristoneura fumiferana NPV (CfMNPV), AcMNPV, and Helicoverpa zea NPV (HzSNPV) were obtained from GenBank. Comparisons of the predicted amino acid sequences of OpMNPV, CpGV, AcMNPV, CfMNP...Continue Reading

Citations

May 7, 2010·Journal of Virology·Ranran WangZhihong Hu
Jul 30, 2005·Journal of Virology·James M Slavicek, Holly J R Popham
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·S C BraunagelM D Summers
Jan 1, 1996·Cytotechnology·G W Blissard
Jun 13, 2009·Molekuliarnaia genetika, mikrobiologiia i virusologiia·I M Kikhno
Oct 11, 2003·Journal of Biochemistry and Molecular Biology·Kianoush Khajeh RashidanClaude Guertin
Sep 30, 1999·Virology·T HayakawaS Maeda
Feb 11, 2004·The Journal of General Virology·Serafín GutiérrezMiguel López Ferber
May 26, 2005·The Journal of General Virology·Jeffrey M Slack, Susan D Lawrence

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