Characterization of a Highly Virulent Edwardsiella anguillarum Strain Isolated From Greek Aquaculture, and a Spontaneously Induced Prophage Therein

Frontiers in Microbiology
Pantelis KathariosQiyao Wang

Abstract

Edwardsiella-associated outbreaks are increasingly reported on both marine and freshwater aquaculture setups, accounting for severe financial and biomass losses. E. tarda, E. ictaluri, and E. hoshinae have been the traditional causative agents of edwardsiellosis in aquaculture, however, intensive studies due to the significance of the disease have just recently revealed two more species, E. piscicida and E. anguillarum. Whole genome sequencing that was conducted on the strain EA011113, isolated from farmed Diplodus puntazzo after an edwardsiellosis outbreak in Greece, confirmed it as a new clinical strain of E. anguillarum. Extensive phylogenetic analysis showed that this Greek strain is closely related to an Israeli E. piscicida-like clinical strain, isolated from diseased groupers, Epinephelus aeneus and E. marginatus in Red Sea. Bioinformatic analyses of E. anguillarum strain EA011113 unveiled a wide repertoire of potential virulence factors, the effect of which was corroborated by the mortalities that the strain induced in adult zebrafish, Danio rerio, under different levels of infection intensity (LD50 after 48 h: 1.85 × 104 cfu/fish). This strain was non-motile and according to electron microscopy lacked flagella, a fact ...Continue Reading

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Citations

May 28, 2019·Virulence·Ka Yin LeungBupe A Siame
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Datasets Mentioned

BETA
EA181011
PRJNA393918
CP011364.1
ET080813
CP006664.1
CP011359.1
ATCC35051
CP016043.1
EA011113
AAL82718.1

Methods Mentioned

BETA
scanning
transmission electron microscopy
PCR
Restriction-Modification
ubiquitination

Software Mentioned

Edno5
Average Nucleotide Identity
Rapid Annotation Subsystem Technology ( RAST )
ARAGORN
Pathosystems Resource Integration Center platform ( PATRIC )
InterProScan
VF
Masurca assembler
Glimmer
PATRIC

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