Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization

Virology
A Sánchez-FauquierJ A Melero

Abstract

An improved purification procedure for human astrovirus serotype 2 (H-Ast2) has facilitated the isolation of a neutralizing monoclonal antibody (PL-2) directed against one of the major structural proteins (VP26) of H-Ast2. A minor component (VP29) of the virus particles is also recognized by PL-2 antibody. Immunofluorescent staining indicated that VP26 (and/or VP29) has mainly a cytoplasmic location in LLCMK2-infected cells. Immunoelectron microscopy demonstrated that the PL-2 epitope was present in the surface of astrovirus particles. Pulse-chase radiolabeling and immunoprecipitation of H-Ast2-infected cell extracts identified a P86 precursor of VP26. Several intermediate protein species (P74 to P35) that shared the PL-2 epitope were also identified in the infected cells. Finally, partial N-terminal sequencing of VP29 and VP26 polypeptides demonstrated that they originated by alternative processing of P86 after residues 361 and 394, respectively. These results corroborate the location of the astrovirus structural genes at the 3' end of the viral genome included in the previously identified 2.8-kb subgenomic RNA.

Citations

Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jinhui DongYizhi Tao
Jun 30, 2011·Avian Pathology : Journal of the W.V.P.A·D ToddM D Welsh
Apr 21, 2012·Avian Pathology : Journal of the W.V.P.A·V J SmythM D Welsh
May 10, 2013·Journal of Virology·Rebecca M DuBoisStacey Schultz-Cherry
Jan 22, 2000·Journal of Virology·D M Bass, S Qiu
Jul 23, 2002·Journal of Virology·Ernesto MéndezCarlos F Arias
Jul 29, 2004·Journal of Virology·Ernesto MéndezCarlos F Arias
Nov 25, 2003·Journal of Medical Virology·Carlos EspulDavid O Matson
Nov 2, 2011·The Veterinary Clinics of North America. Small Animal Practice·Vito MartellaCanio Buonavoglia
Sep 25, 2010·Journal of Virological Methods·Xiaoyan WangDabing Zhang
Sep 16, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Enrique Royuela, Alicia Sánchez-Fauquier
Jun 21, 2002·The Journal of General Virology·Ute GeigenmüllerSuzanne M Matsui
Apr 12, 2001·The Journal of General Virology·C M JonassenB Grinde
Mar 19, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Maria Cristina MediciAdriana Calderaro
Oct 3, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Vito MartellaSimona De Grazia
Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I WilhelmiA Sánchez-Fauquier
Jun 26, 2001·Journal of Medical Virology·Q H WangH Ushijima
Dec 28, 1999·Microbiology and Immunology·M HachiyaH Ushijima
Jun 21, 2005·Journal of Structural Biology·Jordi Querol-AudíNuria Verdaguer
Jan 4, 2017·Viruses·Shauna A Marvin
Jan 14, 2017·Viruses·Philippe PérotMarc Eloit
Jan 21, 2017·Viruses·Carlos F Arias, Rebecca M DuBois
Jul 29, 2016·Journal of Virology·Yukimatsu TohYizhi J Tao
Dec 18, 2013·Journal of Virology·Ernesto MéndezSusana López
Dec 15, 2015·Journal of Virology·Royce L YorkRebecca M DuBois
Feb 17, 2015·Journal of GLBT Family Studies·Erika L Grafsky
Nov 22, 2000·The Journal of General Virology·M Méndez-TossC F Arias
Jul 19, 2017·Annual Review of Virology·Valerie CortezStacey Schultz-Cherry
Feb 10, 1998·Clinical and Diagnostic Laboratory Immunology·M P KoopmansJ Vinjé

❮ Previous
Next ❯

Related Concepts

Related Feeds