PMID: 9557698Apr 29, 1998Paper

Characterization of a human foamy virus 170-kilodalton Env-Bet fusion protein generated by alternative splicing

Journal of Virology
Dirk Lindemann, A Rethwilm

Abstract

Primate foamy viruses (FVs) express, in addition to the 130-kDa envelope protein, a 170-kDa glycoprotein, which reacts with antisera specific for the envelope and Bel proteins. We determined the exact nature of this 170-kDa glycoprotein by using the molecularly cloned human FV (HFV). Radioimmunoprecipitation analysis of 293T cells transfected with appropriate expression constructs by using antisera specific for the HFV Env, Bel1, and Bel2 proteins, as well as reverse transcription-PCR analysis of HFV-infected cells, demonstrated that this protein is an Env-Bet fusion protein that is secreted into the supernatant. However, it is only loosely associated, or not associated, with viral particles. gp170 is generated by an alternatively spliced Env mRNA using a splice donor and splice acceptor pair localized within the env open reading frame (ORF), which is normally used to generate Bell and Bet transcripts derived from the internal promoter within the env ORF. gp170 is expressed at a level 30 to 50% of the Env precursor gp130. However, it alone does not confer infectivity to HFV particles, because capsids derived from proviruses expressing only the gp170 were not released into the supernatant. In contrast, viruses derived from provi...Continue Reading

References

Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A RethwilmV ter Meulen
May 1, 1990·The Journal of General Virology·K O NetzerV ter Meulen
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S KimD Baltimore
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S R HammesW C Greene
Jan 1, 1986·Annual Review of Genetics·M R Green
Jan 1, 1987·Molecular and Cellular Biology·R B DuBridgeM P Calos
Feb 15, 1985·International Journal of Cancer. Journal International Du Cancer·M NambaT Kimoto
Oct 1, 1994·The Journal of General Virology·H HahnA Rethwilm
Jun 1, 1993·Journal of Virology·M L GironR Emanoil-Ravier
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M LöcheltR M Flügel
Sep 1, 1993·Journal of Virology·G BaunachA Rethwilm
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J X Zou, P A Luciw
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J EnssleA Rethwilm
Mar 21, 1996·Nature·R A Weiss
Jan 1, 1997·Journal of Virology·P A GoepfertM J Mulligan
Apr 1, 1997·Nature Medicine·P W ParrenQ J Sattentau
Aug 1, 1997·The Journal of General Virology·M SchmidtA Rethwilm
Aug 18, 1997·Virology·P D BieniaszM O McClure
Jan 28, 1998·Journal of Virology·N FischerA Rethwilm

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Citations

Jun 20, 2001·Journal of Virology·C D MeieringM L Linial
Jun 20, 2002·Journal of Virology·Charles-Henri LecellierAli Saïb
May 28, 2005·Journal of Virology·Daniel LüfteneggerDirk Lindemann
Dec 28, 2005·Journal of Virology·Frédéric DelebecqueOlivier Schwartz
Jul 1, 2011·Journal of Virology·Erik MüllersDirk Lindemann
Jun 14, 2003·Journal of Virology·Evelina Shikova-LekovaAxel Rethwilm
Mar 9, 2002·Journal of Virology·Charles-Henri LecellierAli Saïb
Aug 8, 2008·Journal of Virology·Annett StangeDirk Lindemann
Sep 28, 2012·Journal of Virology·Réjane RuaAntoine Gessain
Jan 26, 2017·Frontiers in Microbiology·Simon Langer, Daniel Sauter
Oct 15, 2003·Journal of Virology·Martin HeinkeleinAxel Rethwilm
Nov 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christopher D Meiering, Maxine L Linial
Mar 29, 2003·Journal of Virology·Marcus Picard-MaureauDirk Lindemann
Sep 15, 2005·Journal of Virology·Marc CartellieriAxel Rethwilm
Feb 7, 2001·Journal of Virology·J Tobaly-TapieroA Saïb
Mar 12, 1999·Journal of Virology·T PietschmannD Lindemann
Feb 11, 1999·Journal of Virology·M L Linial
Nov 27, 2019·Viruses·Magdalena Materniak-KornasMartin Löchelt
May 18, 2000·Virology·C H Lecellier, A Saïb
Jan 30, 2007·Acta histochemica·Eric J Arnoys, John L Wang

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