Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquito.

Insect Biochemistry and Molecular Biology
Guowu BianJinsong Zhu

Abstract

After female mosquitoes ingest blood from vertebrate hosts, exopeptidases and endopeptidases are required for digesting blood proteins in the midgut into amino acids, which female mosquitoes use to build yolk proteins. These proteases are not always present in the midgut, and their diverse expression patterns suggest that production of these enzymes is highly regulated in order to meet specific physiological demands at various stages. Here we report identification of a serine-type protease, JHA15, in the yellow fever mosquito Aedes aegypti. This protein shares high sequence homology with chymotrypsins, and indeed exhibits specific chymotrypsin enzymatic activity. The JHA15 gene is expressed primarily in the midgut of adult female mosquitoes. Our results indicate that its transcription is activated by juvenile hormone in the newly emerged female adults. Although its mRNA profile is similar to that of the early trypsin gene, we found that JHA15 proteins were readily detected in the midgut epithelium cells of both non-blood-fed and blood-fed mosquitoes. Analysis of polysomal RNA further substantiated that synthesis of JHA15 occurs before and shortly after blood feeding. Knocking down expression of JHA15 resulted in no evident phen...Continue Reading

References

Jan 1, 1977·Journal of Insect Physiology·H H HagedornT R Flanagan
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H H HagedornM K Bohm
Jan 1, 1992·Annual Review of Entomology·A S Raikhel, T S Dhadialla
Mar 1, 1990·General and Comparative Endocrinology·A S Raikhel, A O Lea
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D TzamariasG Thireos
Jul 1, 1973·Journal of Insect Physiology·R W Gwadz, A Spielman
Feb 1, 1982·General and Comparative Endocrinology·J P Shapiro, H H Hagedorn
Feb 1, 1996·Insect Molecular Biology·F G NoriegaM A Wells
Feb 15, 2000·Insect Molecular Biology·M J EdwardsM Jacobs-Lorena
Mar 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yoav AravaDaniel Herschlag
May 29, 2003·Journal of Insect Physiology·F G. Noriega, M A. Wells
Oct 18, 2003·Insect Biochemistry and Molecular Biology·Heather R SandersSarjeet S Gill
Nov 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jinsong ZhuAlexander S Raikhel
Nov 25, 2003·The Journal of Biological Chemistry·Eappen G AbrahamMarcelo Jacobs-Lorena
May 17, 2005·Insect Biochemistry and Molecular Biology·Geoffrey M AttardoAlexander S Raikhel
Feb 14, 2006·Insect Molecular Biology·O MarinottiA A James
Aug 2, 2006·Biochemistry·Martin DevenportMarcelo Jacobs-Lorena
Oct 4, 2006·Molecular and Cellular Biology·Jinsong ZhuAlexander S Raikhel
Jan 31, 2007·BMC Genomics·Emma WarrGeorge Dimopoulos
Apr 13, 2012·Journal of Medical Entomology·B W HarkerF H Collins

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Citations

May 16, 2009·Parasitology Research·Yi-Jie GengRen-Li Zhang
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Meng LiJinsong Zhu
Jan 15, 2014·PLoS Neglected Tropical Diseases·José M C RibeiroPedro L Oliveira
Jun 3, 2014·Parasites & Vectors·Leonardo Saboia-VahiaJose Batista De Jesus
Dec 19, 2009·Insect Biochemistry and Molecular Biology·Jinsong ZhuXing Zhang
Nov 4, 2009·Insect Biochemistry and Molecular Biology·Jun IsoeRoger L Miesfeld
Sep 3, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kiyun Park, Inn-Sil Kwak
Jan 23, 2020·G3 : Genes - Genomes - Genetics·Stephanie GamezOmar S Akbari
Sep 6, 2020·BMC Genomics·Iliano V Coutinho-AbreuJesus G Valenzuela
Nov 15, 2019·Cellular and Molecular Life Sciences : CMLS·Jiasheng Song, Shutang Zhou
Jan 7, 2021·PLoS Neglected Tropical Diseases·Stephanie S de CarvalhoGeorgia C Atella

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