PMID: 7013793Mar 3, 1981Paper

Characterization of a kirromycin-resistant elongation factor Tu from Escherichia coli

Biochemistry
R IvellA Parmeggiani

Abstract

The Escherichia coli strain D2216 contains a kirromycin-resistant elongation factor Tu [EF-Tu(D2216); Fischer, E., Wolf, H., Hantke K., & Parmeggiani, A. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 4341-4345]. This stain grows much more slowly than wild-type E. coli strains and contains less than half the amount of EF-Tu. On isoelectric focusing, the whole cell lysate of strain D2216 as well as pure, crystalline EF-Tu(D2216) comprises only a single species indistinguishable from wild-type EF-Tu. In poly(uridylic acid)- [poly(U)] directed poly(phenylalanine) synthesis, enzymatic binding of aminoacyl transfer ribonucleic acid to the ribosome, and susceptibility to trypsin digestion, EF-Tu(D2216) behaves similarly to the EF-Tu from wild-type strains. Kirromycin, which increases the sensitivity to trypsinization of wild-type EF-Tu, has no effect on mutant EF-Tu. In poly(U)-directed poly(phenylalanine) synthesis, partially trypsinized EF-Tu(D2216) displays a 7-fold reduction of its kirromycin resistance as compared to the intact EF-Tu(D2216). This is approximately 300 times less sensitive to the antibiotic than wild-type EF-Tu. The EF-Tu(D2216), purified and crystallized, exhibits a guanosine 5'-triphosphatase activity in the absence o...Continue Reading

References

Jul 15, 1977·European Journal of Biochemistry·G R Jacobson, J P Rosenbusch
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E FischerA Parmeggiani
Feb 7, 1978·Molecular & General Genetics : MGG·P G Lemaux, D L Miller
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A V Furano
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A Van de KlundertL Bosch
Apr 2, 1979·European Journal of Biochemistry·A Bernardi, F Bernardi
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S PedersenJ D Friesen
Nov 16, 1976·Biochemistry·G R Jacobson, J P Rosenbusch
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A V Furano
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H WolfA Parmeggiani
Jul 1, 1980·European Journal of Biochemistry·A WittinghoferR Leberman
Nov 1, 1980·Electroencephalography and Clinical Neurophysiology·P L ParmeggianiE Perez
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K AraiM Wade

❮ Previous
Next ❯

Citations

Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Dec 3, 1981·Nature·L HudsonA Landy
Feb 15, 1992·European Journal of Biochemistry·M Y MistouA Parmeggiani
Nov 1, 2005·The Journal of Biological Chemistry·Andrea ParmeggianiPoul Nissen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.