PMID: 2484331Jan 1, 1989Paper

Characterization of a large conductance non-selective anion channel in B lymphocytes

Cellular Signalling
F V McCannR J Noelle

Abstract

Studies on ion channel currents in freshly isolated murine B lymphocytes with the patch clamp technique revealed the presence of a non-selective anion channel of large conductance in inside-out (i/o) patches. This channel is characterized here according to its unitary conductance, ion selectivity, regulatory factors, distribution and kinetic behaviour. With a unitary conductance of 348 +/- 4.4 pS in a normal physiological ion gradient, it exhibited an indiscriminate selectivity to cations (Na+ and K+). Selectivity to chloride over sodium was established by substitution of high concentrations of NaCl (450 mM) in the bath (i/o patches), resulting in a selectivity ratio (PCl/PNa) of 33. Selectivity to chloride over potassium was confirmed in a similar manner by substitution of TEA-Cl for KCl, yielding a selectivity ratio (PCl/Pk) greater than 80. Conductance of aspartate through the channel demonstrated the non-selective nature of this anion channel. Voltage proved to be a regulatory factor but other influences on channel activity were also present, including the configuration of the patch (channel is inactive in cell attached patches), and the enhancement of activity at negative membrane voltages by previous pulsing. Intracellula...Continue Reading

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Citations

Apr 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J H RaveslootP J Nijweide
Nov 1, 1992·Pflügers Archiv : European journal of physiology·A Coulombe, E Coraboeuf
Jun 1, 1990·Pflügers Archiv : European journal of physiology·L C SchlichterC Grygorczyk
Mar 1, 1990·The Journal of Membrane Biology·F V McCannR J Noelle
Jun 8, 2006·Pflügers Archiv : European journal of physiology·Joo Hyun NamSung Joon Kim
Apr 12, 2008·Purinergic Signalling·Ravshan Z Sabirov, Yasunobu Okada
Apr 3, 2009·The Journal of Physiological Sciences : JPS·Ravshan Z Sabirov, Yasunobu Okada
Jan 7, 2016·Pflügers Archiv : European journal of physiology·Ravshan Z SabirovYasunobu Okada
Jan 1, 1997·Molecular and Chemical Neuropathology·W G NorthF V McCann
Aug 14, 2019·Immunological Reviews·Trisha Mahtani, Bebhinn Treanor
Jun 11, 2004·American Journal of Physiology. Cell Physiology·Chi-Wai DoMortimer M Civan

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