Characterization of a leucine zipper-containing protein identified by retroviral insertion in avian neuroretina cells.

The Journal of Biological Chemistry
V ProuxMaria Marx

Abstract

We reported previously that post-mitotic chicken embryonic neuroretina (NR) cells are induced to proliferate following in vitro infection with RAV-1, a retrovirus that does not carry an oncogene. NR cell multiplication results from the frequent activation and subsequent retroviral transduction of two related serine/threonine protein kinases, the c-mil/c-raf or c-Rmil/B-raf genes. We also showed that a very early event in the activation of these proto-oncogenes is the synthesis of chimeric mRNAs containing viral and cellular sequences joined by a splicing mechanism. In the current study, we have examined the ability of RAV-1 to induce proliferation of quail NR cells. By using the reverse transcription-polymerase chain reaction technique, we identified, in several proliferating quail NR cultures infected with RAV-1, a chimeric mRNA containing cellular sequences joined to the RAV-1 splice donor site. These cellular sequences are derived from a gene designated R10, which is expressed through a 1.9-kilobase (kb) mRNA detected in several embryonic tissues. A second transcript of 2.3 kb is specifically expressed in the NR, where both transcripts are developmentally regulated. The R10 cDNA encodes a 251-amino acid polypeptide that cont...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G M WahlG R Stark
Jan 1, 1992·Annual Review of Cell Biology·M Kozak
Apr 1, 1992·Current Opinion in Genetics & Development·T Alber
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A SwaroopN Agarwal
May 24, 1991·Science·A LupasJ Stock
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M NishizawaS Kawai
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J GhysdaelD Stehelin
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y MakiP K Vogt
Jul 2, 1973·European Journal of Biochemistry·M Gross-BellardP Chambon
Jul 1, 1984·Cell·I A WilsonR A Lerner
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Dec 11, 1981·Science·F Sanger
Oct 6, 1995·The Journal of Biological Chemistry·J V BarnierG Calothy
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A RehemtullaA Swaroop

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Citations

Oct 12, 2001·Biochemical and Biophysical Research Communications·P SathasivamJ A Byrne
May 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jennifer A ByrneRobert K Bright
Sep 19, 2008·Molecular Carcinogenesis·Lei LiDalin He
Nov 24, 2004·Biochemical and Biophysical Research Communications·Rose BoutrosJennifer A Byrne
Sep 12, 2018·Journal of Cellular Biochemistry·Mihyang HaYun Hak Kim
Jun 30, 2005·International Journal of Cancer. Journal International Du Cancer·Jennifer A ByrneAnna Defazio
Dec 25, 2009·American Journal of Physiology. Cell Physiology·Diana D H ThomasGuy E Groblewski
Jun 1, 2001·The Journal of Biological Chemistry·D D ThomasG E Groblewski
Feb 6, 2004·The Journal of Biological Chemistry·Sayeon ChoByoung-Chul Park
Mar 25, 2017·International Journal of Oncology·Kosuke KatoTatsuo Shirota
Feb 23, 2007·Molecular Cancer Research : MCR·Jennifer D LewisRobert K Bright
Jan 14, 1999·The American Journal of Physiology·G E GroblewskiS A Ernst

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