Characterization of a mannose-6-phosphate isomerase from Geobacillus thermodenitrificans that converts monosaccharides

Biotechnology Letters
Soo-Jin YeomDeok-Kun Oh

Abstract

A recombinant mannose-6-phosphate isomerase from Geobacillus thermodenitrificans (GTMpi) isomerizes aldose substrates possessing hydroxyl groups oriented in the same direction at the C2 and C3 positions such as the D- and L-forms of ribose, lyxose, talose, mannose, and allose. The activity of GTMpi for D-lyxose isomerization was optimal at pH 7.0, 70 degrees C and 1 mM Co(2+). Under these conditions, the k(cat) and K(m) values were 74,300 s(-1) and 390 mM for D-lyxose and 28,800 s(-1) and 470 mM for L-ribose, respectively. The half-lives of the enzyme at 60, 65, and 70 degrees C were 388, 73, and 27 h, respectively. GTMpi catalyzed the conversion of D-lyxose to D-xylulose with a 38% conversion yield after 3 h, and converted L-ribose to L-ribulose with a 29% conversion yield.

References

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Oct 20, 2005·Journal of Bioscience and Bioengineering·Tom Birger GranströmKen Izumori
Dec 26, 2006·Journal of Bacteriology·Eun-Ah ChoYu-Ryang Pyun
Nov 6, 2008·Journal of Biotechnology·Ran-Young YoonDeok-Kun Oh

Citations

Aug 30, 2011·Applied Microbiology and Biotechnology·Chao HuCuiying Hu
Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Koen BeerensWim Soetaert
Jun 15, 2010·Journal of Bioscience and Bioengineering·Hyun-Jung KwonDeok-Kun Oh
Jul 8, 2015·Applied Microbiology and Biotechnology·Wanmeng MuBo Jiang
Jul 10, 2010·Journal of Molecular Biology·Laura M van StaalduinenZongchao Jia
Jun 28, 2016·Applied Microbiology and Biotechnology·Ziwei ChenWanmeng Mu
May 7, 2020·Applied Microbiology and Biotechnology·Jiajun ChenWanmeng Mu
Feb 24, 2020·Applied Microbiology and Biotechnology·Ming ChenWanmeng Mu
Jun 26, 2020·The Journal of Microbiology·Soo-Jung KimSoo-Jin Yeom
Jun 14, 2020·Applied Microbiology and Biotechnology·Jiajun ChenWanmeng Mu

Related Concepts

Bacillaceae
Cobalt
Enzyme Stability
Half-Life
Hydrogen-Ion Concentration
Mannose-6-Phosphate Isomerase
Monosaccharides
Proteins, Recombinant DNA
Substrate Specificity
Enzyme Activators

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