Characterization of a mast cell line that lacks the extracellular domain of membrane c-kit

Immunology
Y A MekoriD D Metcalfe

Abstract

Expression of the c-kit proto-oncogene receptor on mast cells is essential for their normal proliferation and maturation as well as for several biological responses such as chemotaxis and attachment. In the present study we report that the interleukin-3 (IL-3)-dependent mast cell line CFTL-15 lacks the extracellular domain of the c-kit receptor. This observation was made after noting that the c-kit ligand stem cell factor (SCF) could not prevent IL-3 deprivation-induced mast cell apoptosis and that CFTL-15 cells did not proliferate in response to SCF. Flow cytometric analysis employing monoclonal anti-c-kit antibodies, and immunogold labelling with analysis by electron microscopy, subsequently showed a diminished expression of c-kit on CFTL-15 cells. There was no identifiable message for the extracellular domain of c-kit in these cells, as determined by reverse transcriptase-polymerase chain reaction (RT-PCR). These previously unrecognized properties of the CFTL-15 mast cell line allowed the examination of other biological consequences of the lack of c-kit on mast cells. Analysing the ability of these cells to adhere to surface-bound fibronectin, it was found that addition of SCF did not increase their adhesion to this substrat...Continue Reading

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Citations

Dec 26, 2001·The Journal of Experimental Medicine·Z XiangG Nilsson
Jun 11, 2010·Genes and Immunity·J K MoralesJ J Ryan
Jun 12, 2004·American Journal of Respiratory Cell and Molecular Biology·Quan ShaRobert P Schleimer
Jul 26, 2000·Hematology/oncology Clinics of North America·A Kirshenbaum
Sep 15, 2006·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Maria EkoffGunnar Nilsson
Oct 3, 2002·Nature Reviews. Immunology·Toshiaki Kawakami, Stephen J Galli
Dec 20, 2011·Inflammopharmacology·Poliana Ribeiro BarrosoFlaviana Dornela Verli
Jul 25, 2003·Oncogene·Nathalie CasteranPatrice Dubreuil
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Toshiyuki KojimaHajime Karasuyama
Nov 28, 2000·European Journal of Immunology·C LunderiusL Hellman
Aug 26, 2003·The American Journal of Pathology·Karin HartmannBeate M Henz

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