Characterization of a membrane-based gradient generator for use in cell-signaling studies

Lab on a Chip
Vinay V AbhyankarDavid J Beebe

Abstract

This paper describes a method to create stable chemical gradients without requiring fluid flow. The absence of fluid flow makes this device amenable to cell signaling applications where soluble factors can impact cell behavior. This device consists of a membrane-covered source region and a large volume sink region connected by a microfluidic channel. The high fluidic resistance of the membrane limits fluid flow caused by pressure differences in the system, but allows diffusive transport of a chemical species through the membrane and into the channel. The large volume sink region at the end of the microfluidic channel helps to maintain spatial and temporal stability of the gradient. The chemical gradient in a 0.5 mm region near the sink region experiences a maximum of 10 percent change between the 6 and 24 h data points. We present the theory, design, and characterization of this device and provide an example of neutrophil chemotaxis as proof of concept for future quantitative cell-signaling applications.

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Citations

Dec 7, 2013·Molecules and Cells·Morgan Hamon, Jong Wook Hong
Dec 23, 2011·Annals of Biomedical Engineering·Beum Jun Kim, Mingming Wu
Aug 9, 2008·Biomedical Microdevices·Taesung KimMichel M Maharbiz
Apr 4, 2009·Biomedical Microdevices·Ulrike HaesslerMingming Wu
Oct 6, 2009·Biomedical Microdevices·Jianbo ShaoJianlong Zhao
Mar 24, 2010·Biomedical Microdevices·Mark T BreckenridgeHarihara Baskaran
Aug 1, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Gus A WrightChristopher Janetopoulos
Mar 29, 2007·Analytical Chemistry·Bor-han ChuehShuichi Takayama
Oct 22, 2010·Analytical Chemistry·Michelle L KovarikStephen C Jacobson
Sep 8, 2011·Nature Communications·Mohammad A QasaimehDavid Juncker
Dec 17, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Maribella DomenechDavid J Beebe
Jun 6, 2009·Lab on a Chip·Dongshin KimDavid J Beebe
Aug 3, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vijayakrishnan AmbravaneswaranDaniel Irimia
Dec 15, 2010·Journal of Biomechanical Engineering·Qingjun KongMaribel Vazquez
May 11, 2010·Annual Review of Biomedical Engineering·Daniel Irimia
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Jan 8, 2010·International Journal of Molecular Sciences·Ming NiHanry Yu
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Apr 28, 2011·Biomicrofluidics·Huei-Wen WuGwo-Bin Lee
Dec 24, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Xuetao ShiAli Khademhosseini
Apr 15, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Wei-Lun HsuDalton J E Harvie
Jun 4, 2013·Advanced Drug Delivery Reviews·Nam-Trung NguyenDinh-Tuan Phan
Jun 28, 2012·Microfluidics and Nanofluidics·Yandong GaoDeyu Li
Mar 29, 2007·Lab on a Chip·Christopher E Sims, Nancy L Allbritton
Jun 1, 2007·Lab on a Chip·Shing-Yi ChengMingming Wu
Aug 24, 2007·Lab on a Chip·Jacques GoulpeauPatrick Tabeling
Jun 28, 2008·Lab on a Chip·Taekyu KangKi Sung Lee
Jul 25, 2008·Lab on a Chip·Amir ShamlooSarah C Heilshorn
Aug 26, 2009·Lab on a Chip·Javier AtenciaLaurie E Locascio
Apr 2, 2010·Lab on a Chip·Min-Hsien WuGwo-Bin Lee
Jun 18, 2010·Lab on a Chip·Kshitiz GuptaAndre Levchenko
Aug 2, 2011·Lab on a Chip·Jules J VanDersarlNicholas A Melosh
Feb 1, 2013·Lab on a Chip·Avital MendelsonJeremy J Mao
May 29, 2013·Lab on a Chip·Jiandong WuFrancis Lin

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