Abstract
A monoclonal antibody (MAb), 13.3.B4, was obtained from a murine hybridoma after fusing Sp2/0 myeloma cells with spleen cells from C3H mice immunized with mouse L cells transfected with the A1 and B1 genes of HLA-DPw4. In radiobinding-assays, MAb 13.3.B4 bound to HLA transfectants expressing DPw2 or DPw4 as well as DPw2 or DPw4 homozygous B-cell lines, while most cell lines expressing other DP determinants were negative. MAbs with known DP or other HLA class II-specificities were used to inhibit binding of MAb 13.3.B4 in a radioimmunoassay. Three MAbs demonstrated inhibition, but their pattern of reactivity with HLA homozygous B cell lines differed from that of MAb 13.3.B4. An evaluation of DNA sequence data showed that MAb 13.3.B4 reacts with all cell lines expressing DP beta-chains of type 2.1, 2.2, 4.1 or 4.2. No correlation between 13.3.B4-reactivity and expression of DP alpha-chain variants was found. The results indicate that MAb 13.3.B4 defines a polymorphic epitope which may be determined by the sequence gly-gly-pro-met at residues 84-87 of the DP beta-chain.
References
Oct 31, 1978·Biochemistry·M A Markwell, C F Fox
Sep 1, 1988·Human Immunology·J A YoungJ Trowsdale
Jan 3, 1985·Nature·P AustinJ Lamb
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J BodmerJ Young
Mar 11, 1985·Nucleic Acids Research·A Kelly, J Trowsdale
Jul 1, 1985·Immunological Reviews·J TrowsdaleJ Bodmer
Mar 1, 1986·Immunobiology·A ZieglerP Wernet
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J HeyesJ Trowsdale
Jan 1, 1986·Annual Review of Immunology·R N Germain, B Malissen
Jan 1, 1987·Immunogenetics·R E BontropM J Giphart
Jan 1, 1987·Immunogenetics·N TanigakiJ Cooper
Oct 1, 1969·British Journal of Plastic Surgery·C C Knowles
Apr 16, 1981·Nature·L M NadlerJ M Pesando
Oct 1, 1983·Tissue Antigens·T MoenE Thorsby
Nov 11, 1982·Nucleic Acids Research·F G GrosveldR A Flavell
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Citations
Nov 1, 1990·Human Immunology·G Mueller-EckhardtC Mueller-Eckhardt
Oct 1, 1995·Human Immunology·H D VikenE Thorsby
Apr 1, 1995·Tissue Antigens·H D VikenG Gaudernack
Nov 14, 1997·The Journal of Experimental Medicine·S P FlingD Pious
Oct 12, 2014·Human Immunology·Rene J DuquesnoySemiramis J H do Monte
Feb 17, 1998·European Journal of Immunology·A M RasmussenS Funderud
Sep 25, 2016·Human Immunology·Daimon P SimmonsIndira Guleria
Apr 7, 1994·Nature·S P FlingD Pious