Characterization of a mouse cortical collecting duct cell line

Kidney International
B A StoosG Fejes-Tóth


A cortical collecting duct (CCD) cell line has been developed from a mouse transgenic for the early region of simian virus 40, Tg(SV40E)Bri/7. CCDs were microdissected and placed on collagen gels. Monolayers were subsequently subcultured onto permeable collagen membranes and maintained in serum-supplemented medium. One line, designated M-1, retained many characteristics of the CCD, including a typical epithelial appearance and CCD-specific antigens. M-1 cells, when grown in monolayers on permeable supports, exhibited a high transepithelial resistance (885.7 +/- 109.6 ohms/cm2) and developed a lumen negative transepithelial potential difference (PD) of -45.7 +/- 3.5 mV. The associated short-circuit current (SCC) averaged 71.8 +/- 10.3 microA/cm2, and was reduced by 95% by luminal application of amiloride. The cultured cells responded to arginine vasopressin (AVP) with a significant increase in SCC. M-1 cells generated significant transepithelial solute gradients. After 24 hours incubation, the composition of the luminal (L) and basolateral (B) media (in mM) was: [Na+], L = 106.7 +/- 0.9 and B = 127.4 +/- 0.4; [K+], L = 8.6 +/- 0.6 and B = 2.1 +/- 0.3; [Cl], L = 68.6 +/- 5.8 and B = 101.8 +/- 6.6; [HCO3], L = 15.5 +/- 1.5 and B =...Continue Reading


Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J S HandlerJ P Johnson
Sep 1, 1979·Kidney International·J R Hoyer, M W Seiler
Feb 1, 1986·The American Journal of Physiology·S Sariban-Sohraby, D J Benos
Aug 1, 1986·The American Journal of Physiology·J D Valentich, M F Stokols
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L AlperD Brown
Jan 1, 1988·Renal Physiology and Biochemistry·G J Gstraunthaler
Sep 1, 1986·The American Journal of Physiology·V L SchusterM L Jennings
Oct 1, 1968·The American Journal of Physiology·M B BurgJ Orloff
Jul 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·C R GaushT F Smith
Sep 1, 1981·The American Journal of Physiology·F M Perkins, J S Handler

❮ Previous
Next ❯


Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·G Fejes-Tóth, A Náray-Fejes-Tóth
Feb 1, 1996·In Vitro Cellular & Developmental Biology. Animal·C A RankinJ P Calvet
Nov 2, 2004·Pflügers Archiv : European journal of physiology·Gabriele AdamKarl Kunzelmann
Sep 20, 2005·Pflügers Archiv : European journal of physiology·Charity NofzigerBonnie L Blazer-Yost
Aug 29, 2006·Pflügers Archiv : European journal of physiology·M BensA Vandewalle
Apr 23, 2008·Pflügers Archiv : European journal of physiology·M Bens, A Vandewalle
Aug 7, 2012·Pflügers Archiv : European journal of physiology·Silke HaerteisChristoph Korbmacher
Nov 15, 2005·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Roger G O'NeilNizar Mullani
Jan 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·M E Rafestin-OblinA Vandewalle
Oct 22, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Ekaterina PetrovichHaim Garty
Jan 29, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Woon Kyu LeeHitoshi Endou
Jul 28, 2001·Kidney International·M SuttersJ P Calvet
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jianghui HouSiegfried Waldegger
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Fejes-Tóth, A Náray-Fejes-Tóth
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·I AhmadC J Barnstable
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·T VolkC Korbmacher
Apr 27, 2012·The Journal of Biological Chemistry·Shiri MoshitzkyHaim Garty
Mar 24, 2009·The Journal of Biological Chemistry·Il-Ha LeeAnuwat Dinudom
Dec 14, 1999·Current Opinion in Nephrology and Hypertension·A VandewalleJ P Duong Van Huyen
Jun 10, 2009·Molecular and Cellular Biology·Tadahiro KitamuraDomenico Accili
Nov 9, 2012·American Journal of Physiology. Cell Physiology·Daria V IlatovskayaAlexander Staruschenko
May 28, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ke LiYanbin Dong
Dec 16, 2011·American Journal of Physiology. Renal Physiology·Emily BorstingRobyn Cunard
Feb 18, 2012·American Journal of Physiology. Renal Physiology·Kirk L Hamilton, Daniel C Devor
Apr 21, 2012·Journal of the American Society of Nephrology : JASN·Juan DuXiaoqiang Yao
Dec 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Anikó Náray-Fejes-TóthGéza Fejes-Tóth
Oct 24, 1997·In Vitro Cellular & Developmental Biology. Animal·S ZannoniC Asselin
Feb 1, 1995·Kidney International·P BriandA Vandewalle
Jan 24, 2012·Biochemical and Biophysical Research Communications·Rania NasrallahRichard L Hébert
May 4, 2010·Molecular and Cellular Endocrinology·Victoria McEneaneyWarren Thomas
Oct 7, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Victoria McEneaneyWarren Thomas
Apr 8, 2009·The American Journal of Pathology·Johannes SchödelCarsten Willam

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.