Characterization of a murine alpha 2 macroglobulin gene expressed in reproductive and cardiovascular tissue

Biology of Reproduction
Hong HeB A Croy

Abstract

Full-length cDNA for a mouse gene A2-macroglobulin induced by pregnancy (A2mp) was cloned from mesometrial decidua at Gestation Day 10. The 4622-base pair cDNA encodes a protein of 1473 AA with >70% sequence identity and all typical domains of other A2M-family members in humans and rodents, despite unique absence of hepatic expression. The bait region is most distinct and has the greatest sequence similarity with rat acute-phase A2m. Northern blotting, reverse transcription and real-time-PCR, and in situ hybridization studies using C57Bl/6 mice revealed uterine induction of A2mp during decidualization and strong, midgestational association with modifying spiral arteries. Ovaries, testes, lactating mammary glands, heart, and kidney were the only additional organs with A2mp expression that was localized to granulosa and cumulus cells in secondary follicles; primary seminiferous epithelium, including Sertoli cells, mammary alveolar, and ductal epithelium; cardiac endothelium; and renal collecting tubules, respectively. Infusion of native human A2M into pregnant alymphoid or interferon-gamma gene-ablated mice overcame blocks to pregnancy-induced spiral artery modification in these strains. Activated human A2M was also effective, su...Continue Reading

References

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Citations

Jul 5, 2012·Endocrinology·Kristin E BurnumSudhansu K Dey
Mar 27, 2015·Thrombosis and Haemostasis·Ricardo Rodríguez-CalvoJosé Martínez-González
Jun 25, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jennifer L Herington, Brent M Bany
Nov 22, 2005·Molecular and Cellular Endocrinology·Chandrakant TayadeB A Croy
Aug 17, 2005·Atherosclerosis·Ponnambalam AnnapooraniGovindan Sadasivam Selvam
Aug 19, 2005·Journal of Reproductive Immunology·Marianne van den HeuvelB Anne Croy
Oct 25, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Matthew T RätsepB Anne Croy
Nov 15, 2006·Immunological Reviews·B Anne CroyChandrakant Tayade
Jun 17, 2020·Scientific Reports·Daniel Bastida-RuizMarie Cohen
Feb 21, 2008·Reproductive Biomedicine Online·M J BilinskiB A Croy

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Datasets Mentioned

BETA
AY185125

Methods Mentioned

BETA
PCR

Software Mentioned

Clustal W
Optimas
RelQuant LightCycler
EnSembl

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