PMID: 108276Jun 10, 1979

Characterization of a mutant form of ribosomal protein S1 from Escherichia coli.

The Journal of Biological Chemistry
A R Subramanian, S Mizushima

Abstract

An altered form of ribosomal protein S1 from a mutant of Escherichia coli has been isolated and characterized. The mutant protein (denoted m1-S1) has a molecular weight of 57,000 as shown by sodium dodecyl sulfate-gel electrophoresis and the same NH2-terminal sequence as wild type S1. Protein m1-S1 binds poly(U) in the same manner as protein S1 and is active in protein synthesis with either synthetic or natural mRNA. Thus, about 75% of the sequence of protein S1 (which includes the NH2-terminal region) contains essentially all the functional domains of this protein involved in protein biosynthesis.

Related Concepts

Cross Reactions
SDS-PAGE
Alkalescens-Dispar Group
Immunodiffusion Measurement
Kinetics
Mutation
Poly U
Ribosomal Proteins
Protein Biosynthesis

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