Characterization of a naturally processed MHC class II-restricted T-cell determinant of hen egg lysozyme.

Nature
S DemotzA Sette

Abstract

Compelling evidence indicates that T cells recognize complexes formed by major histocompatibility complex-encoded molecules and antigenic peptide fragments. This is based largely on the ability of small synthetic peptides to substitute for naturally processed antigen in stimulating T cells. Naturally processed fragments of exogenous antigen are thought to arise by limited proteolytic degradation of native antigen inside acidic compartments of antigen-presenting cells, but until now no physiologically processed antigen has been directly analysed. Here we report the characterization of physiologically processed antigen eluted from mouse class II major histocompatibility complex I-Ed molecules. The antigenic material corresponds to a previously described antigenic determinant of hen egg lysozyme (HEL 107-116) and has a relative molecular mass Mr of about 2,000. HPLC analysis identified at least two or three separate molecular species, suggesting limited, albeit significant, heterogeneity of naturally processed peptides. Finally, under our experimental conditions, it was calculated that a substantial proportion (10-40%) of I-Ed molecules were occupied by these HEL-derived antigenic determinants.

References

Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L AdoriniE Appella
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S BuusH M Grey
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H Z StreicherJ A Berzofsky

❮ Previous
Next ❯

Citations

Nov 6, 1990·Journal of Immunological Methods·R Busch, J B Rothbard
Mar 15, 1993·Journal of Immunological Methods·T D BrumeanuH Zaghouani
Jun 1, 1991·Research in Immunology·M HadzijaT L Delovitch
Feb 1, 1991·Current Opinion in Immunology·C V Harding
Feb 1, 1992·Current Opinion in Immunology·A Sette, H M Grey
Feb 1, 1993·Current Opinion in Immunology·H G RammenseeO Rötzschke
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates
Sep 29, 1999·Immunology Today·M BelloneM P Protti
May 1, 1997·Human Immunology·S C Schneider, E E Sercarz
Feb 1, 1996·Current Opinion in Immunology·E Goulmy
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K Dornmair, H M McConnell
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M SrinivasanS K Pierce
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S DemotzH M Grey
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R CibottiP Kourilsky
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A NelsonE R Unanue
Nov 1, 1991·The Journal of Experimental Medicine·O RötzschkeH G Rammensee
Jun 24, 2004·Infection and Immunity·Kristina M Williams, Elmer C Bigley
Oct 11, 1992·The Journal of Clinical Investigation·M P ProttiB M Conti-Tronconi
Aug 1, 1993·The Journal of Clinical Investigation·A A ManfrediB M Conti-Tronconi
Nov 24, 1999·The Journal of Clinical Investigation·M PeakmanR M Chicz
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·R R LatekE R Unanue
Nov 1, 1991·European Journal of Immunology·O RötzschkeH G Rammensee
Feb 1, 1991·European Journal of Immunology·E MottezP Kourilsky
Aug 1, 1992·European Journal of Immunology·V BhardwajE E Sercarz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.