Characterization of a neuronal cell line expressing native human melanin-concentrating hormone receptor 1 (MCHR1)

The International Journal of Biochemistry & Cell Biology
Dennis FryChristine A Collins

Abstract

Melanin-concentrating hormone (MCH), an orexigenic neuropeptide in mammals, activates a G-protein coupled receptor, MCHR1. It is expected that antagonists of MCHR1 function will prove therapeutically useful as anti-obesity agents. Intracellular signaling by MCHR1 has been investigated primarily using non-neural cell lines expressing the recombinant receptor, in which MCHR1 has been shown to couple to G alpha(i/o) and G alpha(q) G-proteins. While these cell lines have been widely utilized to discover and optimize small molecule antagonists, it is unknown whether the intracellular signaling pathways in these cells accurately reflect those in neurons. Thus, we sought to develop a neurally derived cell line endogenously expressing MCHR1. IMR32, a human neuroblastoma cell line, has been shown to express MCHR1 mRNA; however, we were unable to detect either MCH-binding or MCH-stimulated Ca++-mobilization in these cells. Following transfection of IMR32 cells with a plasmid encoding human G alpha(16) G-protein, we isolated a cell line, I3.4.2, which responded to MCH in Ca++-mobilization assays. We found that the expression level of MCHR1 mRNA in I3.4.2 cells was 2000-fold higher than in the parent cell line. Using [125I]MCH saturation-b...Continue Reading

References

Jan 1, 1999·Nature·M ShimadaE Maratos-Flier
Aug 12, 1999·Biochemical and Biophysical Research Communications·Y ShimomuraM Fujino
Sep 21, 2000·Trends in Endocrinology and Metabolism : TEM·Y SaitoO Civelli
Feb 13, 2001·The Journal of Clinical Investigation·D S LudwigE Maratos-Flier
Mar 29, 2001·The Journal of Biological Chemistry·J HillA I Muir
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A W SailerQ Liu
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S AnK Dai
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y SaitoK Maruyama
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Donald J MarshSu Qian
Aug 17, 2002·Peptides·Steven FriedBrian E Hawes
Nov 9, 2002·Anatomical Science International·Hitoshi KawanoShinji Hayashi
Nov 26, 2002·Biochimica Et Biophysica Acta·Sonja L Toutenhoofd, Emanuel E Strehler
Jan 30, 2003·American Journal of Physiology. Endocrinology and Metabolism·Masahiko ItoAkio Kanatani
Jun 27, 2003·Trends in Endocrinology and Metabolism : TEM·Pavlos Pissios, Eleftheria Maratos-Flier
Sep 5, 2003·European Journal of Pharmacology·Lauren P ShearmanAlison M Strack

❮ Previous
Next ❯

Citations

May 5, 2011·Regulatory Peptides·Akie HamamotoYumiko Saito
Feb 23, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Eduardo D Gigante, Tamara Caspary
Nov 19, 2010·Journal of Receptor and Signal Transduction Research·Alex N EberleUrs Zumsteg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.