Characterization of a new beta(1-3)-glucan branching activity of Aspergillus fumigatus.

The Journal of Biological Chemistry
Amandine GasteboisThierry Fontaine

Abstract

A new HPLC method was developed to separate linear from beta(1-6)-branched beta(1-3)-glucooligosaccharides. This methodology has permitted the isolation of the first fungal beta(1-6)/beta(1-3)-glucan branching transglycosidase using a cell wall autolysate of Aspergillus fumigatus (Af). The encoding gene, AfBGT2 is an ortholog of AfBGT1, another transglycosidase of A. fumigatus previously analyzed (Mouyna, I., Hartland, R. P., Fontaine, T., Diaquin, M., Simenel, C., Delepierre, M., Henrissat, B., and Latgé, J. P. (1998) Microbiology 144, 3171-3180). Both enzymes release laminaribiose from the reducing end of a beta(1-3)-linked oligosaccharide and transfer the remaining chain to another molecule of the original substrate. The AfBgt1p transfer occurs at C-6 of the non-reducing end group of the acceptor, creating a kinked beta(1-3;1-6) linear molecule. The AfBgt2p transfer takes place at the C-6 of an internal group of the acceptor, resulting in a beta(1-3)-linked product with a beta(1-6)-linked side branch. The single Afbgt2 mutant and the double Afbgt1/Afbgt2 mutant in A. fumigatus did not display any cell wall phenotype showing that these activities were not responsible for the construction of the branched beta(1-3)-glucans of t...Continue Reading

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Citations

Jun 15, 2010·Eukaryotic Cell·Amandine GasteboisIsabelle Mouyna
Jan 13, 2016·Applied Biochemistry and Biotechnology·Kristína Kováčová, Vladimír Farkaš
Jul 9, 2013·Microbiology·Jolanda M van MunsterMarc J E C van der Maarel
Dec 15, 2015·Molecular Plant Pathology·Longzhou Liu, Stephen J Free
Dec 6, 2019·Microbiology and Molecular Biology Reviews : MMBR·Tim J H BaltussenWillem J G Melchers
Nov 10, 2012·The Journal of Biological Chemistry·Jakob EngelFrançoise H Routier
Aug 8, 2015·Acta Crystallographica. Section D, Biological Crystallography·Zhen QinShiwang Wu
Feb 28, 2016·The Journal of Microbiology·Mark J Lee, Donald C Sheppard
Mar 15, 2014·The Journal of Biological Chemistry·Thomas StalhbergerThierry Fontaine
Apr 12, 2019·Nature Communications·Wenxia FangDaan M F van Aalten
Sep 11, 2020·Traffic·Miguel A PeñalvaMiguel Hernández-González
Mar 13, 2017·Fungal Genetics and Biology : FG & B·Jie Ao, Stephen J Free
Aug 18, 2021·Journal of Plant Physiology·Yanxin WangZhongli Cui

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