Characterization of a new brain-derived proteoglycan inhibiting retinal ganglion cell axon outgrowth

Molecular and Cellular Neurosciences
S Henke-FahleP P Monnier

Abstract

A proteoglycan was identified and isolated from physiological saline extracts of chick embryo brains by using a new monoclonal antibody (hybridoma clone mab Te38). The purified proteoglycan displayed an apparent molecular mass of 2500-3500 kDa, which became reduced to 370 and 600 kDa after digestion with chondroitinase ABC or chondroitinase AC. After additional treatment with keratanase the 600-kDa band was no longer detectable in Western blots. The specific epitope recognized by mab Te38 is an O-linked carbohydrate associated with the core protein. Tenascin-C, an extracellular matrix protein known to associate with several proteoglycans, copurified with the mab Te38 proteoglycan on the immunoaffinity column. Mab Te38 binds to the surface of nonneuronal cells; in sections from the primary visual system, expression is restricted to cells in the optic fissure, the dorsal optic nerve, and the chiasm. No retinal cells were found to express the mab Te38 epitope. The isolated molecule inhibited axon outgrowth from retinal explants when offered bound to a substrate consisting of either matrigel or collagen, chondroitinase treatment did not alter the inhibitory properties. The distribution and in vitro function of the Te38 proteoglycan...Continue Reading

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Citations

Jul 22, 2008·Neurobiology of Disease·Kenneth MinorStephen J Davies
Jul 20, 2005·Progress in Retinal and Eye Research·Glen Jeffery, Lynda Erskine
Oct 14, 2014·BioMed Research International·Justin R SiebertDonna J Osterhout
Dec 18, 2013·The Analyst·Chen-Chun ChenRofeamor P Obena

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