Characterization of a new endo-type polysaccharide lyase (PL) family 6 alginate lyase with cold-adapted and metal ions-resisted property

International Journal of Biological Macromolecules
Shan GaoFeng Han

Abstract

Alginate lyase played an important role in brown algae degradation, and its enzymatic degradation products showed various biological activities. Although many alginate lyases have been characterized, the enzymes with special characterizations are still rather rare. In this study, a new alginate lyase gene, tsaly6A, has been cloned from marine bacterium Thalassomonas sp. LD5, and expressed in Escherichia coli. The deduced alginate lyase, TsAly6A, belonged to the polysaccharide lyase (PL) family 6 and showed the highest amino acid identity (63%) with an exo-type oligoalginate lyase AlyGC. However, this study showed that TsAly6A was an endo-type enzyme yielding alginate trisaccharides (64.5%) as the main products. Compared with other alginate lyases, TsAly6A showed high trisaccharide-yielding levels. Meanwhile, TsAly6A showed the specific activity of 15,960 U/μmol at its optimal pH (pH 8.0) and temperature (35 °C). In addition, TsAly6A was a cold-adapted, salt-activated and metal ions-resisted alginate lyase, which will enable it to perform high activity in the solution containing various ions. Its cold-adaptation, metal ions-tolerance and high trisaccharides yields make TsAly6A an excellent candidate for industrial applications.

References

Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·K Iwasaki, Y Matsubara
May 6, 2003·Bioscience, Biotechnology, and Biochemistry·Yoshiko IwamotoTsuyoshi Muramatsu
Dec 6, 2005·Journal of Industrial Microbiology & Biotechnology·Michelle E RauchMorley O Stone
Nov 13, 2008·Extremophiles : Life Under Extreme Conditions·Tohru KobayashiKoki Horikoshi
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
May 17, 2013·Journal of Microbiology and Biotechnology·Akihiro SakatokuShogo Nakamura
Nov 20, 2014·PloS One·Steven M SwiftDaniel C Nelson
Nov 19, 2017·Applied and Environmental Microbiology·Fei XuXiu-Lan Chen
Apr 24, 2018·Journal of Bioscience and Bioengineering·Manman HeChagan Irbis

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Citations

Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Jun LiuZhengqiang Jiang
Mar 2, 2021·Acta Crystallographica. Section D, Structural Biology·Keke ZhangQianqian Lyu
Aug 4, 2020·International Journal of Biological Macromolecules·Danyang ChengXiangzhao Mao
Oct 11, 2020·International Journal of Biological Macromolecules·Yujiao LiFeng Han
Jun 14, 2021·Carbohydrate Polymers·Ushasree Mrudulakumari VasudevanEun Yeol Lee
Jul 5, 2021·International Journal of Biological Macromolecules·Tahreem RiazJingjing Chen
Jul 14, 2021·Critical Reviews in Food Science and Nutrition·Chunhua ZhangChunpeng Wan

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