Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China

Archives of Virology
Kuanyu ZhengJiahong Dong

Abstract

A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80-120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively. This is the first report of complete sequences of PCSV in mainland China. Phylogenetic analysis of all tospoviral proteins indicated that PCSV-14YV733 is closely related to members of the WSMoV serogroup.

References

Mar 6, 2008·Archives of Virology·Jia-Hong DongZhong-Kai Zhang
Nov 26, 2010·Archives of Virology·Channarong SeepibanSupat Attathom
Jun 12, 2012·Advances in Virus Research·Massimo TurinaMarina Ciuffo
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Dec 4, 2014·Virology Journal·Yueyan YinZhongkai Zhang
Mar 10, 2015·Nature Genetics·Hideki NishikawaHaruhiko Fujiwara

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Citations

Dec 1, 2018·Current Opinion in Virology·Dorith Rotenberg, Anna E Whitfield

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Methods Mentioned

BETA
transmission electron microscopy
RNA-seq
PCR
electrophoresis
ELISA

Software Mentioned

Platanus
MEGA
DNAMAN
ClustalW

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