Characterization of a new model of thromboembolic stroke in C57 black/6J mice.

Translational Stroke Research
Saema AnsarStephen Meairs

Abstract

This study characterizes a new model of thromboembolic stroke of the middle cerebral artery in C57 black/6J mice, thus offering an opportunity to use the model for studying ischemic stroke in transgenic mice. Thromboembolic stroke was induced by local injection of either 1.5 or 3.0 UI of thrombin directly into the right MCA of C57 black/6J mice. Cerebral blood flow (CBF) velocity was measured continuously by laser Doppler flowmetry, which allowed documentation of both MCA occlusion and of spontaneous recanalization. After 24 h, all animals were euthanized. Cryosections were cut at 400-μm intervals and silver stained with the high-contrast method for volumetric assessment of infarct size. Interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), caspase-3 and hsp 70 protein levels were investigated by immunofluorescence. Thrombin injection resulted in clot formation in all animals. Cortical infarction occurred in 63% of the mice while 37% had a spontaneous MCA recanalization during the first 20 min following thrombin injection. In cases of successful MCA occlusion with consequent infarction, the clot was stable up to 2 h after formation. Subsequently, 20% recanalized spontaneously. Infarctions were restricted to the cortex with a...Continue Reading

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Citations

Feb 24, 2016·Brain Research Bulletin·Anil KumarVarun Gupta
Apr 1, 2016·Thrombosis and Haemostasis·Frederik Denorme, Simon F De Meyer
Sep 20, 2015·Journal of Neuroscience Methods·Yingxin ChenNabil J Alkayed
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Feb 20, 2018·Translational Stroke Research·Nagarajesh GorlamandalaGary D Housley
Jun 5, 2018·Zeitschrift für Psychosomatische Medizin und Psychotherapie·Jochen HardtWilfried Laubach
Apr 15, 2020·Translational Stroke Research·Huiting ZhangXiaotang Ma
Oct 6, 2020·Brain, Behavior, and Immunity·Damien LevardMarina Rubio
Feb 19, 2021·Journal of Stroke·Dmitri NikitinDong-Eog Kim

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Methods Mentioned

BETA
confocal microscopy
transgenic

Software Mentioned

ImageJ

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