PMID: 2504924Jul 20, 1989Paper

Characterization of a new prokaryotic transcriptional activator and its DNA recognition site

Journal of Molecular Biology
I Barthelemy, M Salas

Abstract

The expression of the Bacillus subtilis phage phi 29 DNA is controlled by the viral gene 4 product, which is required for the initiation of transcription at the unique late promoter A3. Protein p4 binds specifically to a phi 29 DNA fragment containing the A3 promoter. DNase I footprinting analysis has shown that the DNA binding region for protein p4 is located between nucleotides -50 and -100 relative to the transcription start site. Methylation interference assays suggest that two eight base-pair long inverted repeats located within this binding region are the protein p4 recognition sequence. These results, together with the fact that the protein p4-dependent in vitro transcription requires the B. subtilis sigma 43-RNA polymerase, indicate that protein p4 is a transcriptional activator. The protein p4 DNA recognition region is statically bent as suggested by gel retardation and chemical cleavage assays. A model of protein p4 binding to its DNA target site is proposed.

References

Jan 1, 1986·Gene·Y MizukamiH Hirokawa
Dec 1, 1986·Journal of Virology·I BarthelemyR P Mellado
May 1, 1987·Journal of Virology·I BarthelemyR P Mellado
Aug 26, 1986·Nucleic Acids Research·M Gribskov, R R Burgess
Sep 20, 1986·Journal of Molecular Biology·R P MelladoM Salas
Dec 26, 1986·Cell·H N Liu-JohnsonD M Crothers
Apr 10, 1986·Nature·H S KooD M Crothers
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Oct 15, 1976·Virology·M R InciarteE Viñuela

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Citations

Sep 1, 1991·Research in Microbiology·F RojoM Salas
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B NuezM Salas
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MencíaM Salas
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MonsalveF Rojo
Jan 12, 2005·Nucleic Acids Research·Laura Pérez-LagoAna Camacho
Sep 18, 2007·Journal of Bacteriology·Martín AlcorloJosé M Hermoso
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·W J MeijerM Salas
Jan 1, 2010·International Journal of Molecular Sciences·Ana Camacho, Margarita Salas
Apr 24, 2010·FEMS Microbiology Reviews·Ana Camacho, Margarita Salas
May 13, 1999·Journal of Bacteriology·F Rojo
Jan 1, 1991·Journal of Bacteriology·G B Van BokkelenR Calendar
Jun 1, 1994·Microbiological Reviews·J Pérez-MartínV de Lorenzo
May 25, 1990·Science·M SerranoJ M Hermoso

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