Characterization of a non-human primate model for the study of testicular peritubular cells-comparison with human testicular peritubular cells

Molecular Human Reproduction
N SchmidArtur Mayerhofer

Abstract

Are monkey testicular peritubular cells (MKTPCs) from the common marmoset monkey (Callithrix jacchus) a suitable translational model for the study of human testicular peritubular cells (HTPCs)? MKTPCs can be isolated and propagated in vitro, retain characteristic markers for testicular peritubular cells and their proteome strongly (correlation coefficient of 0.78) overlaps with the proteome of HTPCs. Smooth-muscle-like peritubular cells form the wall of seminiferous tubules, transport sperm, are immunologically active, secrete a plethora of factors and may contribute to the spermatogonial stem cell niche. Mechanistic studies are hampered by heterogeneity of human samples. We established a culture method for MKTPCs and characterized these cells from six young adult animals (2-3 years). To examine whether they qualify as a translational model we also examined HTPCs from seven men and compared the proteomes of both groups. We used explant cultures to obtain MKTPCs, which express smooth muscle markers (calponin (CNN1), smooth muscle actin (ACTA2)), lack FSH-receptors (FSHR) and LH-receptors (LHCGR), but possess androgen receptors (AR). MKTPCs can be passaged at least up to eight times, without discernable phenotypic changes. Mass-s...Continue Reading

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Citations

Mar 31, 2019·Biology of Reproduction·Sadman SakibIna Dobrinski
Feb 19, 2019·Cell Death Discovery·Konstantin BagnjukArtur Mayerhofer
Oct 23, 2019·Scientific Reports·Nina SchmidArtur Mayerhofer
Mar 7, 2021·International Journal of Molecular Sciences·Nina SchmidArtur Mayerhofer

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