Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta

Journal of Neuroinflammation
Ryan C McCarthyMarianne Wessling-Resnick

Abstract

Alzheimer's disease is associated with amyloid-beta (Aβ)-induced microglia activation. This pro-inflammatory response promotes neuronal damage, and therapies are sought to limit microglial activation. Screening efforts to develop new pharmacological inhibitors require a robust in vitro cell system. Current models lack significant responses to Aβ, and their use in examining age-related neurodegenerative diseases is questionable. For example, the commonly used BV-2 microglial line was derived from embryonic mononuclear cells and its activation by various stimuli is limited. To this end, we have established a new immortalized microglial (IMG) cell line from adult murine brain. The objective of this study was to characterize Aβ-induced activation of IMG cells, and here, we demonstrate the ability of cannabinoids to significantly reduce this inflammatory response. Microglial cells derived from adult murine brain were immortalized via infection with the v-raf/v-myc retrovirus under conditions that selectively promote microglia growth. The presence or absence of markers CD11b and F4/80 (microglial), NeuN (neuronal), and GFAP (astrocytic) was assessed by immunofluorescence microscopy and western blotting. Using IMG and BV-2 cells, leve...Continue Reading

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Citations

Jul 30, 2016·Frontiers in Immunology·Dusica Maysinger, Issan Zhang
Mar 18, 2017·Frontiers in Immunology·Carole SousaAlessandro Michelucci
Apr 4, 2018·The Journal of Biological Chemistry·Ryan C McCarthyMarianne Wessling-Resnick
Sep 30, 2016·The Biochemical Journal·Young Ah SeoMarianne Wessling-Resnick
Nov 7, 2019·Journal of Neurochemistry·Israel C NnahMarianne Wessling-Resnick
Jun 16, 2018·Experimental and Therapeutic Medicine·Qi ZengDaxiang Lu
Aug 22, 2018·Frontiers in Cellular Neuroscience·Raissa TimmermanJeffrey J Bajramovic

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Methods Mentioned

BETA
flow cytometry
PCR
ELISA
dot blot
fluorescence microscopy

Software Mentioned

ImageJ
Zeiss AxioVision
FlowJo
Prism GraphPad
GraphPad

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