Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua)

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Mar Marcos-LopezHugh W Ferguson

Abstract

Alloherpesviruses affect freshwater and marine fish species. The aim of the current study was to characterize a novel alloherpesvirus in Atlantic cod (Gadus morhua). Samples were processed for histopathology, transmission electron microscopy (TEM), virus isolation, molecular characterization, and in situ hybridization (ISH). Histopathology revealed that the infection was restricted to the gills and that it induced cytomegaly in infected cells. By TEM, numerous viral particles with morphology compatible with a herpesvirus were observed inside the cytomegalic cells. To characterize this new agent, polymerase chain reaction amplified regions of the ATPase subunit of the terminase, and DNA polymerase genes were sequenced. Phylogenetic analysis revealed strongest similarity with alloherpesviruses belonging to the genus Ictalurivirus and Salmonivirus. The ISH showed specific labeling of nuclear inclusions in the cytomegalic cells. While virus isolation was unsuccessful, the results obtained through different diagnostic tests in the present study confirm the discovery of a new alloherpesvirus affecting Atlantic cod. The authors propose the formal species designation Gadid herpesvirus 1 (GaHV-1) to be considered for approval by the Int...Continue Reading

References

Jan 1, 1992·Virology·A J Davison
Oct 1, 1971·Journal of Virology·K Wolf, R W Darlington
Apr 22, 1999·The Journal of General Virology·B EhlersM Goltz
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Sep 4, 2001·The Veterinary Record·W G StarkeyH W Ferguson
Sep 12, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S Essbauer, W Ahne
Nov 2, 2001·Journal of Virology·L M IyerE V Koonin
Jul 3, 2002·Diseases of Aquatic Organisms·H HellbergI Bjerkås
Mar 25, 2004·The Journal of General Virology·Bernhard Ehlers, Stewart Lowden
Dec 9, 2004·Diseases of Aquatic Organisms·Dong Lim ChoiMi Seon Park
Dec 23, 2004·Journal of Virology·Duncan J McGeoch, Derek Gatherer
Jan 22, 2005·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
May 26, 2005·The Journal of General Virology·Thomas B WaltzekRonald P Hedrick
Sep 1, 1978·Journal of Virology·K WolfT Nagabayashi
Oct 25, 2006·Diseases of Aquatic Organisms·Ole B SamuelsenOivind Bergh
Nov 14, 2006·The Journal of General Virology·Andrew J DavisonRobert G McKinnell
Nov 24, 2007·Journal of Fish Diseases·S MiwaM Sano
Dec 11, 2008·Archives of Virology·Andrew J DavisonEtienne Thiry
Jul 2, 2009·Diseases of Aquatic Organisms·Thomas B WaltzekRonald P Hedrick
Dec 18, 2009·The Journal of General Virology·Steven J van BeurdenMarc Y Engelsma
Mar 1, 2000·Journal of Aquatic Animal Health·R P HedrickA Eldar
Jan 1, 1993·Annual Review of Fish Diseases·Frank M Hetrick, Ronald P Hedrick

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Citations

Dec 14, 2011·Viruses·Larry HansonMoshe Kotler
Jan 16, 2014·Virusdisease·Arun K DharF C Thomas Allnutt
Jan 17, 2015·Science China. Life Sciences·QiYa Zhang, Jian-Fang Gui
Jun 5, 2018·Diseases of Aquatic Organisms·Kyle A GarverE Tellervo Valtonen
Dec 3, 2016·Diseases of Aquatic Organisms·Jared T FreitasThomas B Waltzek

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Methods Mentioned

BETA
PCR
transmission
electrophoresis

Software Mentioned

BLASTP
Mafft
ClustalW
MrBayes

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