Characterization of a novel ArsR-like regulator encoded by Rv2034 in Mycobacterium tuberculosis.

PloS One
Chun-hui GaoZheng-Guo He

Abstract

The genome of Mycobacterium tuberculosis, the causative agent of tuberculosis, encodes a large number of putative transcriptional regulators. However, the identity and target genes of only a few of them have been clearly identified to date. In a recent study, the ArsR family regulator Rv2034 was characterized as a novel positive regulator of phoP. In the current study, we characterized the auto-repressive capabilities of Rv2034 and identified several residues in the protein critical for its DNA binding activities. We also provide evidence that Rv2034 forms dimers in vitro. Furthermore, by using DNaseI footprinting assays, a palindromic sequence was identified as its binding site. Notably, we found that the dosR promoter region contains the binding motif for Rv2034, and that Rv2034 positively regulates the expression of the dosR gene. The potential roles of Rv2034 in the regulation of lipid metabolism and hypoxic adaptation are discussed.

References

Jun 1, 1991·Molecular Microbiology·J Wu, B P Rosen
Jun 6, 1991·Nature·C K StoverG F Hatfull
Dec 26, 2001·Nucleic Acids Research·Alex BatemanErik L L Sonnhammer
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Isabelle VergneVojo Deretic
Nov 13, 2004·Trends in Microbiology·Sharon L KendallNeil G Stoker
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Feb 14, 2006·FEMS Microbiology Reviews·Antonio J Molina-HenaresJuan L Ramos
Mar 8, 2007·Nature Medicine·Ann M Ginsberg, Melvin Spigelman
Jun 26, 2007·Journal of Bacteriology·Rosicler L Barbosa, Celso E Benedetti
Nov 28, 2007·Nucleic Acids Research·Robert D FinnAlex Bateman
Jan 31, 2008·PloS One·Tige R RustadDavid R Sherman
May 14, 2008·Cell Host & Microbe·Michael U ShilohJeffery S Cox
Sep 2, 2008·Journal of Bacteriology·Jesús Gonzalo-AsensioCarlos Martín
Oct 7, 2008·Nucleic Acids Research·T B K ReddyGary K Schoolnik
Mar 14, 2009·Current Opinion in Microbiology·Anne O Summers
Apr 25, 2009·Cellular Microbiology·Tige R RustadDavid R Sherman
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alphonse I ArunkumarDavid P Giedroc
Nov 17, 2009·Journal of Bacteriology·Kevin P O'RourkeGrace A Spatafora
Nov 21, 2009·Microbiology·Haiyan ZhaoKottayil I Varughese
May 22, 2010·Tuberculosis·James E GalaganGary K Schoolnik
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Jun 10, 2011·Nucleic Acids Research·Santosh ChauhanJaya Sivaswami Tyagi

❮ Previous
Next ❯

Citations

Sep 27, 2015·Microbiology·Deepak RudrappaPaul Blum
Jun 30, 2016·Journal of Biomolecular Structure & Dynamics·Qiming LiJianping Xie
Mar 13, 2014·Molecular & Cellular Proteomics : MCP·Cuauhtemoc Licona-CassaniLars K Nielsen
Oct 19, 2017·Journal of Industrial Microbiology & Biotechnology·Xu-Ming MaoYong-Quan Li
Dec 6, 2016·Acta Crystallographica. Section F, Structural Biology Communications·R Alexandra BajajLinda Miallau
Jul 5, 2013·Nature·James E GalaganGary K Schoolnik
May 18, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rudra P SahaManoj Kumar Singh
Apr 1, 2014·Microbiology Spectrum·Suma JainiJames Galagan
Feb 1, 2014·Microbiology Spectrum·Tanya Parish

❮ Previous
Next ❯

Methods Mentioned

BETA
one-hybrid
electrophoresis
footprinting
ChIP-seq
electrophoretic mobility shift
PCR

Software Mentioned

pJEM

Related Concepts

Related Feeds

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.