Characterization of a novel biosurfactant produced by marine hydrocarbon-degrading bacterium Achromobacter sp. HZ01

Journal of Applied Microbiology
M-C DengJ-H Wang

Abstract

To purify and characterize the biosurfactants produced by Achromobacter sp. HZ01. After fermentation, one biosurfactant was successfully purified from the fermentation broth of strain HZ01 by centrifugation, extraction using ethyl acetate, silica gel chromatography and reversed phase-high performance liquid chromatography. The critical micelle concentration (CMC) of the biosurfactant and the effects of temperatures, pH and salinities on its stability were determined. Fourier transform infrared spectroscopy, analysis of fatty acids and amino acids and mass spectrometry were used to characterize the biosurfactant. The maximum production yield of the crude biosurfactant reached to 6·84 g l(-1) after incubation for 96 h. Except the favourable adaptability to a wide range of temperatures, pH and salinities, the biosurfactant with a CMC value of 48 mg l(-1) could efficiently emulsify diverse hydrophobic compounds. The chemical formula of this biosurfactant was confirmed to be CH3 -(CH2 )17 -CHO-CH2 -CO-Gly-Gly-Leu-Met-Leu-Leu, in which the oxygen atom of group CHO linked to the last amino acid (Leu), a structure had never been reported before. The purified biosurfactant is a novel cyclic lipopeptide. One novel lipopeptide was purifie...Continue Reading

References

Nov 5, 1997·Biologicals : Journal of the International Association of Biological Standardization·D VollenbroichG Pauli
Dec 31, 1998·Antimicrobial Agents and Chemotherapy·A J De Lucca, T J Walsh
Feb 7, 2001·Journal of Clinical Microbiology·S E KayM M Peel
May 17, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J Gómez-CerezoJ J Vázquez
Dec 6, 2003·International Journal of Systematic and Evolutionary Microbiology·Tom CoenyePeter Vandamme
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Jun 1, 2005·Journal of Natural Products·Torsten NeuhofHans von Döhren
Feb 1, 1987·Applied and Environmental Microbiology·D G Cooper, B G Goldenberg
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira
May 3, 2006·Journal of Biochemical and Biophysical Methods·Shi-Zhong YangBo-Zhong Mu
Sep 26, 2006·Trends in Biotechnology·Soumen MukherjeeRamkrishna Sen
Sep 2, 2008·Bioresource Technology·Palashpriya DasRamkrishna Sen
Dec 25, 2009·Journal of Natural Products·Marcy J BalunasWilliam H Gerwick
Feb 23, 2010·Biotechnology Advances·Surekha K SatputeBalu A Chopade
Apr 29, 2010·Applied Microbiology and Biotechnology·Ibrahim M BanatRoger Marchant
Apr 5, 2011·Colloids and Surfaces. B, Biointerfaces·Muthu Irulappan SriramSangiliyandi Gurunathan
Jan 16, 2013·Biotechnology Advances·Santi M MandalOctavio L Franco
Dec 5, 2014·Saudi Journal of Biological Sciences·Yesurethinam SarafinThavasimuthu Citarasu
Jan 30, 2015·BioMed Research International·Khem Raj Meena, Shamsher S Kanwar

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Citations

Oct 17, 2018·Journal of Mass Spectrometry : JMS·Nawel JemilMoncef Nasri
Aug 30, 2017·Frontiers in Microbiology·Yue-Hui HongJiang-Hai Wang
Jan 15, 2020·Applied Biochemistry and Biotechnology·Abdullahi Adekilekun Jimoh, Johnson Lin
Aug 27, 2018·Biotechnology Letters·Lakshmi TripathiIbrahim M Banat
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Samadhan WaghmodeSurekha K Satpute
Jul 19, 2019·Journal of Hazardous Materials·Hari RamSyed G Dastager
Dec 3, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Somayeh KazemzadehMasoud Fouladgar
Jan 12, 2021·Saudi Journal of Biological Sciences·Adegoke Isiaka Adetunji, Ademola Olufolahan Olaniran

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