Characterization of a Novel blaKLUC Variant With Reduced β-Lactam Resistance From an IncA/C Group Plasmid in a Clinical Klebsiella pneumoniae Isolate

Frontiers in Microbiology
Pingping LiTeng Xu

Abstract

Similar to other CTX-M family enzymes, KLUC is a recently identified and emerging determinant of cefotaxime resistance that has been recovered from at least three Enterobacteriaceae species, including Kluyvera cryocrescens, Escherichia coli, and Enterobacter cloacae. Whether this extended-spectrum β-lactamase (ESBL) has been disseminated among commonly isolated Enterobacteriaceae is worthy of further investigation. In this study, we screened 739 nosocomial Enterobacteriaceae isolates (240 Klebsiella pneumoniae and 499 E. coli strains) and found that one K. pneumoniae and four E. coli isolates harbored the blaKLUC gene. Three blaKLUC determinants isolated from E. coli were entirely identical to a blaKLUC-3 gene previously recovered in the same hospital. PFGE of four blaKLUC-harboring E. coli strains showed that prevalence of these determinants was most likely mediated by horizontal gene transfer but not clonal dissemination. However, the variant isolated from K. pneumoniae belonged to a novel member of the KLUC enzyme group. This newly identified enzyme (KLUC-5) has an amino acid substitution compared with previously identified KLUC-1 (G18S) and KLUC-3 (G240D). Antimicrobial susceptibility tests showed that KLUC-5 significantly ...Continue Reading

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Citations

Sep 3, 2020·International Journal of Environmental Research and Public Health·Guoying WangHongju Wang

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Datasets Mentioned

BETA
MH476540
KM670336
BCTM01000044
EF219134
EF382672
KJ406378
CP017058.1

Methods Mentioned

BETA
Assay
PCR
electrophoresis

Software Mentioned

BLASTX
Genome Analysis Toolkit
Canu
MAFFT
MODEL
SWISS
Bwa
MEGA6
BioNumerics
Glimmer

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