Characterization of a novel carotenoid cleavage dioxygenase from plants

The Journal of Biological Chemistry
Steven H SchwartzJan A D Zeevaart

Abstract

The plant hormone abscisic acid is derived from the oxidative cleavage of a carotenoid precursor. Enzymes that catalyze this carotenoid cleavage reaction, nine-cis epoxy-carotenoid dioxygenases, have been identified in several plant species. Similar proteins, whose functions are not yet known, are present in diverse organisms. A putative cleavage enzyme from Arabidopsis thaliana contains several highly conserved motifs found in other carotenoid cleavage enzymes. However, the overall homology with known abscisic acid biosynthetic enzymes is low. To determine the biochemical function of this protein, it was expressed in Escherichia coli and used for in vitro assays. The recombinant protein was able to cleave a variety of carotenoids at the 9-10 and 9'-10' positions. In most instances, the enzyme cleaves the substrate symmetrically to produce a C(14) dialdehyde and two C(13) products, which vary depending on the carotenoid substrate. Based upon sequence similarity, orthologs of this gene are present throughout the plant kingdom. A similar protein in beans catalyzes the same reaction in vitro. The characterization of these activities offers the potential to synthesize a variety of interesting, natural products and is the first step...Continue Reading

References

Dec 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J A Olson, N I Krinsky
Oct 4, 1996·The Journal of Biological Chemistry·Z SunF X Cunningham
Jun 20, 1997·Science·S H SchwartzD R McCarty
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B C TanD R McCarty
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·A BurbidgeI B Taylor
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Qin, J A Zeevaart
Nov 28, 2000·The Journal of Biological Chemistry·T M RedmondF X Cunningham
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C D Rock, J A Zeevaart
Sep 16, 1966·Science·T Eisner, J Meinwald

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Citations

Apr 17, 2010·Planta·Michael H WalterDieter Strack
Aug 23, 2012·Journal of Industrial Microbiology & Biotechnology·Christoph NackeJens Schrader
Oct 23, 2013·Archives of Biochemistry and Biophysics·Peter J Harrison, Timothy D H Bugg
Nov 11, 2003·Trends in Plant Science·Ottoline Leyser
Apr 9, 2005·Trends in Plant Science·Alexander R MoiseKrzysztof Palczewski
Apr 9, 2005·Trends in Plant Science·Florence BouvierBilal Camara
Apr 25, 2003·Phytochemistry·Peter FleischmannPeter Winterhalter
Dec 24, 2002·Biochimica Et Biophysica Acta·Steven H SchwartzJan A D Zeevaart
Jan 24, 2002·Trends in Plant Science·Mitsunori Seo, Tomokazu Koshiba
Apr 25, 2003·Trends in Plant Science·Giovanni GiulianoJohannes von Lintig
Jun 1, 2002·Current Opinion in Chemical Biology·Matthew J Ryle, Robert P Hausinger
Nov 17, 2012·Chemical Reviews·Roman Bielski, Zbigniew Witczak
Nov 2, 2013·Chemical Reviews·Alexander R MoiseEleanore T Wurtzel
Jun 18, 2011·Journal of Agricultural and Food Chemistry·Rockendra GuptaV M Balasubramaniam
Aug 27, 2011·Journal of Agricultural and Food Chemistry·Mark A SeftonDennis K Taylor
Jun 19, 2013·Journal of Agricultural and Food Chemistry·Camilla Z GallonTimothy J O'Hare
Jul 10, 2008·The Journal of Biological Chemistry·Angela RubioLourdes Gómez-Gómez
Dec 23, 2008·The Journal of Biological Chemistry·Martin J SergeantAndrew J Thompson
May 14, 2009·Journal of Experimental Botany·Fong-Chin HuangWilfried Schwab
Jul 14, 2012·Journal of Experimental Botany·Bianyun YuAbdelali Hannoufa
Mar 17, 2012·Science·Adrian AlderSalim Al-Babili
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Daisuke UmenoFrances H Arnold
Jun 5, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Shukui GuoMichèle C Loewen
Nov 22, 2011·BMC Plant Biology·Sabine GuillaumieSerge Delrot

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