Characterization of a novel chronic photothrombotic ring stroke model in rats by magnetic resonance imaging, biochemical imaging, and histology

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Thomas HilgerPer Wester

Abstract

A novel photothrombotic ring stroke model was characterized by multiparametric magnetic resonance imaging, imaging of cerebral blood flow (CBF), adenosine triphosphate (ATP), pH, and histology. Ischemia was initiated by transosseous irradiation of a predefined brain area intravenously perfused by the photosensitive dye erythrosin B in male Wistar rats. In the region of the primary ring-lesion, the phototoxic reaction caused necrosis reflected by low relative ATP levels (28 +/- 15%), alkalosis (pH: 7.35 +/- 0.50), and histologic evidence at 14 days after lesion induction. In the ring-encircled interior region (region-at-risk), spontaneous tissue reperfusion (relative CBF: 93 +/- 3%) enabled partial tissue preservation. This was demonstrated by a less impaired energy metabolism (ATP: 65 +/- 23%), normal pH (7.01 +/- 0.50), and still normal cellular structures shown by histologic staining. Analysis of the temporal characteristics within the region-at-risk revealed a slow continuous increase of the apparent diffusion coefficient of water (ADC) to 144 +/- 16% of control (14d) and an early vasogenic edema, reflected by an increase of the T2 relaxation time to 143 +/- 17% of control (2d). Both final ADC and T2 correlated well with the...Continue Reading

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Citations

Feb 13, 2010·Experimental & Translational Stroke Medicine·Kenneth M Sicard, Marc Fisher
Nov 18, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wei JiangPer Wester
May 25, 2007·Pharmacology, Biochemistry, and Behavior·Aysan Durukan, Turgut Tatlisumak
May 23, 2012·Experimental Neurology·Jamshid FarajiGerlinde A Metz
Mar 28, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Richard LeighPeter Cm van Zijl
Dec 1, 2017·Translational Stroke Research·Anatoly B Uzdensky
Jun 21, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Xiao-Fei HeZhong Pei
Oct 23, 2019·International Journal of Molecular Sciences·Sarah Lunardi Baccetto, Christian Lehmann
Feb 19, 2021·Journal of Stroke·Dmitri NikitinDong-Eog Kim

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