Characterization of a novel fungal chitosanase Csn2 from Gongronella sp. JG

Carbohydrate Research
Jun WangYujuan Wang

Abstract

A 28kDa chitosanase designated as Csn2 was purified from the culture broth of the fungus Gongronella sp. JG through three chromatography steps: CM-Sepharose FF, Superdex 200 and SP-Sepharose FF. Its optimal reaction pH and temperature were pH 5.6 and between 55 degrees C and 60 degrees C. The half-lives of Csn2 at 50 degrees C and 55 degrees C were estimated to be 30min and 11min, respectively. The K(m) value of Csn2 in sodium acetate buffer (pH 5.6) at 55 degrees C was 8.86mg/mL. Mn(2+), Ca(2+) and Sr(2+) were activators of Csn2; ETDA was an inhibitor. Cu(2+) stimulated Csn2 at 1mM, but inhibited Csn2 activity at 10mM. Csn2 displayed strong activity on colloidal chitosan, but did not hydrolyze colloidal chitin and carboxylmethyl cellulose. Thin layer chromatography analysis showed the end products of colloidal chitosan hydrolyzed by Csn2 were chitobiose, chitotriose and chitotetraose with chitotriose as the major product. The N terminus of Csn2 was determined to be YQLPANLKKIYDSHKSGTC. Part of the genomic DNA sequence corresponding to Csn2 was cloned. Sequence alignment showed DNA sequence of Csn2 was partly identical to chitosanase genes from Metarhizium anisopliae var. acridum, Hypocrea lixii and Aspergillus fumigatus. Based...Continue Reading

References

Jan 1, 1988·Microbiology and Immunology·A TokoroM Suzuki
Feb 1, 1996·Nature Structural Biology·E M MarcotteJ D Robertus
Nov 24, 1999·Current Microbiology·M KurakakeT Komaki
Dec 15, 2000·Biotechnology and Applied Biochemistry·C Y Cheng, Y K Li
Oct 29, 2002·The Biochemical Journal·Anne Colomer-PallasRégis Chambert
May 6, 2003·Bioscience, Biotechnology, and Biochemistry·Toshiaki TanabeMasaru Mitsutomi
Oct 7, 2004·Journal of Molecular Biology·Wataru AdachiAkio Takénaka
Sep 10, 2005·Applied and Environmental Microbiology·ChoongSoo YunMakoto Kawamukai
Nov 8, 2005·Journal of Biochemistry·Tamo FukamizoAkikazu Ando
Dec 7, 2005·The Journal of Biological Chemistry·Chih-Yu ChengYaw-Kuen Li
Feb 1, 1995·Applied and Environmental Microbiology·M ShimosakaM Okazaki
Apr 29, 2006·Applied Microbiology and Biotechnology·Y S LeeY L Choi
Jun 8, 2006·Molecular Biotechnology·Chasanah EkowatiMaggy Thenawidjaja Suhartono
Apr 19, 2008·Carbohydrate Research·San-Lang WangKao-Cheng Liu

❮ Previous
Next ❯

Citations

Nov 26, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mariana Gabriela GhinetRyszard Brzezinski
Jan 23, 2015·Marine Drugs·Tzu-Wen LiangSan-Lang Wang
Dec 24, 2013·Food Chemistry·Nidheesh Thadathil, Suresh Puthanveetil Velappan
Feb 1, 2015·Applied Biochemistry and Biotechnology·Zhanping ZhouJiangning Song
Jan 19, 2019·World Journal of Microbiology & Biotechnology·Gleb E AktuganovVioletta R Safina
Oct 16, 2019·Frontiers in Bioengineering and Biotechnology·Michal Benedykt KaczmarekMaurycy Daroch
Mar 16, 2021·Frontiers in Microbiology·Qiuling ZhengXuehong Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.