Characterization of a novel galactan produced by Weissella confusa KR780676 from an acidic fermented food

International Journal of Biological Macromolecules
Digambar KavitakePrathapkumar Halady Shetty

Abstract

An exopolysaccharide (EPS) producing strain PUFSTM055 isolated from Idli batter (an Indian traditional cereal-legume based fermented food) was identified as Weissella confusa KR780676. The strain was shown to produce 17.2g/L (dry weight) of EPS in 2% sucrose supplemented MRS broth and the EPS was characterized. HPTLC analysis confirmed the presence of galactose monomers, indicating the homopolysaccharide nature of EPS. Fourier-transform infrared spectroscopy and nuclear magnetic resonance analysis revealed that the EPS was found to be a novel linear galactan containing α-(1→6)-linked galactose units. Scanning electron microscopy of the EPS revealed the presence of porous and spongy starch-like granules. Topographical examination of EPS by atomic force microscopy revealed that the EPS formed densely packed mesh-like structure with irregular spherical lumps. The EPS also showed high thermal stability with a degradation temperature of 287.5 °C and melting point at 274.65 °C. EPS was semi-crystalline with crystallinity index of 0.23 and showed 100% water solubility index. These characteristics of the EPS would make it a promising hydrocolloid for food industries as bio-thickeners, stabilizers and also as an encapsulating material f...Continue Reading

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Citations

Apr 19, 2019·Journal of Food Science and Technology·Digambar KavitakePrathapkumar Halady Shetty
Feb 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan LiLu Wen
Mar 18, 2020·Journal of Food Science and Technology·Digambar KavitakePrathapkumar Halady Shetty
Jul 25, 2019·Environmental Science and Pollution Research International·Sivagnanam SilambarasanVelu Rajesh Kannan
Dec 16, 2020·Microbial Biotechnology·Esperanza Martínez-RomeroLuis Eduardo Servín-Garcidueñas
Feb 11, 2021·Probiotics and Antimicrobial Proteins·Camila Gonçalves TeixeiraAntônio Fernandes de Carvalho
Jan 23, 2021·International Journal of Biological Macromolecules·Ghoson M DabaWaill A Elkhateeb
Mar 3, 2021·International Journal of Biological Macromolecules·Dan ZhaoJingping Ge
Apr 6, 2021·Frontiers in Microbiology·Diana Hernández-OaxacaAgustín López Munguía
Jan 18, 2018·International Journal of Biological Macromolecules·A T Adesulu-DahunsiK Banwo
May 12, 2019·International Journal of Biological Macromolecules·Digambar KavitakePrasad Andhare
May 31, 2019·International Journal of Biological Macromolecules·Digambar KavitakePrathapkumar Halady Shetty
May 28, 2021·International Journal of Biological Macromolecules·Swati TiwariPrathapkumar Halady Shetty
May 17, 2021·Food Research International·Palanisamy Bruntha DeviPrathapkumar Halady Shetty

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