Characterization of a novel gene, wosA, regulating FlhDC expression in Proteus mirabilis

Journal of Bacteriology
Janet K Hatt, Philip N Rather

Abstract

In this study, we describe wosA, a Proteus mirabilis gene identified by its ability to increase swarming motility when overexpressed. At various times during the swarming cycle, the increased expression of wosA resulted in a 4- to 16-fold upregulation of the transcription of flhDC, encoding the master regulator of the flagellar cascade. In turn, the expression of flaA, encoding flagellin, was substantially increased in wosA-overexpressing strains. The overexpression of wosA also resulted in constitutive swarmer cell differentiation in liquid medium, a normally nonpermissive condition. However, in wosA-overexpressing strains, the onset of swarming was not altered. A null wosA allele resulted in a slight decrease in swarming motility. The expression of wosA was growth phase dependent during growth in liquid and on agar plates during swarmer cell differentiation. Increasing the viscosity of liquid medium by the addition of polyvinylpyrrolidone induced swarmer cell differentiation and resulted in a fourfold increase in wosA transcription. A fliL mutation that results in constitutive swarmer cell elongation also increased wosA transcription. In this study, we discuss the possible role of the wosA gene product in signal transduction ...Continue Reading

References

Oct 1, 1992·Journal of Bacteriology·W ShiC A Gross
Oct 1, 1991·Journal of Bacteriology·R BelasD Flaherty
Aug 1, 1990·Journal of Bacteriology·L Alberti, R M Harshey
Apr 1, 1989·Molecular & General Genetics : MGG·A Nishimura, Y Hirota
Jul 1, 1986·Journal of Bacteriology·R BelasM Silverman
Jan 1, 1974·Archives of Microbiology·S Ulitzur
Dec 1, 1972·Bacteriological Reviews·J Henrichsen
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·R M Harshey, T Matsuyama
Feb 1, 1996·Journal of Bacteriology·B M Prüss, P Matsumura
Nov 1, 1996·Journal of Bacteriology·O RauprichJ A Shapiro
Dec 23, 1999·Current Opinion in Microbiology·G M Fraser, C Hughes
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·G S Chilcott, K T Hughes
Jun 12, 2001·Methods in Enzymology·M Alavi, R Belas
Jan 5, 2002·Journal of Medical Microbiology·S J LiawW B Wang
Jul 17, 2003·Molecular Microbiology·Anne Francez-CharlotKaymeuang Cam
Oct 7, 2003·Annual Review of Microbiology·Rasika M Harshey
Feb 6, 2004·Molecular Microbiology·Gwen Sturgill, Philip N Rather
Jun 25, 2004·Nucleic Acids Research·Aron Marchler-Bauer, Stephen H Bryant
Jul 14, 2005·Environmental Microbiology·Philip N Rather
Sep 13, 2005·Annual Review of Microbiology·Nadim Majdalani, Susan Gottesman
Dec 15, 2005·Nucleic Acids Research·Haeyoung JeongJihyun F Kim
Sep 19, 2006·Journal of Bacteriology·Lindsay G Stevenson, Philip N Rather

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Citations

Oct 9, 2012·Nature Reviews. Microbiology·Chelsie E Armbruster, Harry L T Mobley
Apr 7, 2010·Infection and Immunity·Melanie M PearsonHarry L T Mobley
Apr 1, 2008·Journal of Bacteriology·Melanie M PearsonHarry L T Mobley
Nov 28, 2009·Trends in Microbiology·Jennifer K AndersonTimothy R Hoover
Sep 9, 2008·Trends in Microbiology·Natalie VerstraetenJan Michiels
May 26, 2010·FEMS Microbiology Reviews·Randy M MorgensteinPhilip N Rather
Jun 16, 2010·FEMS Microbiology Letters·Deborah A SiegeleWeimin Mao
Nov 7, 2015·Microbiology Spectrum·Jessica N Schaffer, Melanie M Pearson

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