Oct 1, 1989

Characterization of a novel human respiratory syncytial virus chimeric FG glycoprotein expressed using a baculovirus vector

The Journal of General Virology
M W WathenB R Murphy


Human respiratory syncytial virus (RSV) codes for two glycoproteins (F and G) which have been shown to the major targets for the host antibody response. We have expressed a novel chimeric glycoprotein (FG) in insect cells using a baculovirus vector. The chimeric glycoprotein contains the signal and extracellular regions of the RSV F glycoprotein linked to the extracellular region of the RSV G glycoprotein. Beginning at the amino terminus, the chimeric glycoprotein consists of amino acids 1 to 489 from RSV F followed by amino acids 97 to 279 from RSV G. The chimeric FG glycoprotein did not contain an anchor region and was efficiently secreted into the medium of recombinant baculovirus-infected insect cells. The FG glycoprotein ranged in size from 69K to 91K and was heterogeneous with respect to isoelectric point. The cleavage site present on the F glycoprotein was recognized on the chimeric FG, and the glycoprotein appeared to be antigenically similar to the native RSV F and G glycoproteins.

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Mentioned in this Paper

Respiratory Syncytial Virus Infections
Precipitin Tests
Shuttle Vectors
Chimeric Proteins, Recombinant
Post-Translational Protein Processing
Amino Acids, I.V. solution additive
Human respiratory syncytial virus
Antigenic Specificity

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