Characterization of a novel interaction between vasodilator-stimulated phosphoprotein and Abelson interactor 1 in human platelets: a concerted computational and experimental approach

Arteriosclerosis, Thrombosis, and Vascular Biology
Marcus DittrichIngvild Birschmann

Abstract

The goal of this study was systematic profiling of vasodilator-stimulated phosphoprotein (VASP)-Ena/VASP homology 1 (EVH1) interactors in human platelets using a combined in silico and in vitro approach. Exploiting the information of the comprehensive proteome catalogue in the PlateletWeb database (http://plateletweb.bioapps.biozentrum.uni-wuerzburg.de/PlateletWeb.php), we performed a motif search of all sequences and identified potential target sites of class I EVH1 domains in human platelet proteins. Performing affinity purification with VASP-EVH1 domain and the lysates of platelets, we examined complex partners by mass spectrometry. Combining the results of both analyses, we identified Abelson interactor 1 (Abi-1) as a novel EVH1 domain-specific interaction partner of VASP in human platelets and investigated this interaction by yeast 2-hybrid mutational studies and immunoprecipitation. Immunofluorescence microscopy indicated colocalization of both proteins at the lamellipodia of spread human platelets, suggesting a role in reorganizing the cytoskeleton during spreading. The combination of experimental and computational interactome research has emerged as a valuable tool for the analysis of protein-protein interaction network...Continue Reading

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Citations

Nov 30, 2011·Blood·Desislava BoyanovaMarcus Dittrich
Oct 31, 2014·Molecular Biology of the Cell·Svitlana HavrylenkoJulie Plastino
Sep 10, 2014·Developmental Cell·Xing Judy ChenMichael Way
Aug 6, 2014·Proteomics. Clinical Applications·Christina Yip, Angel García

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