Characterization of a novel microsomal fatty acid synthetase (FAS) compared to a cytosolic FAS in the housefly, Musca domestica

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
P GuG J Blomquist

Abstract

A novel membrane-bound fatty acid synthetase (FAS) associated with the microsomal fraction from the housefly, Musca domestica, was solubilized and purified to homogeneity. The microsomal FAS was solubilized by 0.75 M KCl in phosphate buffer and was purified to homogeneity by the sequential use of ammonium sulfate precipitation followed by Sepharose CL-6B, DEAE Sephacel and Red Agarose (dye ligand affinity) chromatography. The specific activity of the microsomal FAS was increased 1,440-fold to 6,522 U/mg during purification. The cytosolic FAS from the housefly was also purified by similar methods and the specific activity increased 183-fold to 7,533 U/mg. The relative molecular mass of the microsomal and cytosolic FAS are 419 +/- 22 kDa and 405 +/- 18 kDa, respectively, for the dimers as determined by gel permeation chromatography. The microsomal and the cytosolic FAS yield different tryptic digestion maps and have slightly different amino acid compositions, which demonstrate structural differences between the two FASs. In addition, there are differences between the two FASs in their kinetic characteristics and their ability to incorporate methylmalonylCoA into the growing fatty acyl chain.

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Citations

Jul 16, 1999·Insect Biochemistry and Molecular Biology·J A TillmanG J Blomquist
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eckhart Schweizer, Jörg Hofmann
Sep 10, 2004·Annual Review of Entomology·Ralph W Howard, Gary J Blomquist
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jan E BelloJocelyn G Millar
Jul 27, 2004·Archives of Insect Biochemistry and Physiology·M Patricia Juárez
Oct 19, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M P Juárez, G C Fernández
May 20, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Henry Chung, Sean B Carroll
Jun 17, 2015·Progress in Lipid Research·Joanne Y Yew, Henry Chung
Aug 23, 2006·Cell·Soo Hee LeePaul T Englund
May 21, 2013·Current Opinion in Chemical Biology·Jillian L BlattiMichael D Burkart
Jun 10, 2020·Nature Communications·Joshua P TorresEric W Schmidt
Mar 18, 2021·G3 : Genes - Genomes - Genetics·Philipp P SprengerBarbara Feldmeyer

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