Abstract
It is generally accepted that live attenuated influenza vaccine (LAIV) has the potential for use as a vaccination against flu. In this study, we demonstrated the nature of an influenza A virus (IAV) mutant induced by treating the IAV with a stable furan derivative, (1R,2R)-1-(5'-methylfur-3'-yl)propane-1,2,3-triol (MFPT), which had been isolated from Streptomyces sp. strain FV60 with the objective of it being an LAIV candidate. The resulting MFPT-resistant (MFPTr) IAVs possessed attenuated pathogenicity in vitro and in vivo when compared with that of the parent virus (H1N1 subtype, NWS strain). Sequencing analysis revealed that a novel mutation, C490U in ns gene (P164S in NS1), was detected in all MFPTr virus clones tested. Therefore, NS1 might be a main target of MFPT, and it was suggested that the P164S mutation contributed to the attenuated pathogenicity of the mutants. Although the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway is one of the targets of NS1, the MFPTr virus suppressed the phosphorylation of Akt when compared with the wild-type (WT) virus. It was suggested that this might lead to the subsequent inhibition of the cleavage of PARP-1 and caspase-3, which is important for the progression of apoptosis....Continue Reading
References
Aug 10, 2000·The Journal of Antimicrobial Chemotherapy·K HayashiT Hayashi
Jan 29, 2002·Archives of Virology·E HoffmannD R Perez
Apr 25, 2002·Nucleic Acids Research·Michael W PfafflLeo Dempfle
May 30, 2003·The EMBO Journal·Walter J WurzerStephan Ludwig
Aug 6, 2005·Journal of Clinical Microbiology·Shuji HatakeyamaYoshihiro Kawaoka
Apr 21, 2006·Nature·Hiroki KatoShizuo Akira
Nov 4, 2006·Virus Research·Sébastien BouloFlorence Baudin
Dec 16, 2006·The Journal of General Virology·Yeun-Kyung ShinYan Zhou
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ronan Le GofficMustapha Si-Tahar
Sep 21, 2007·Journal of Virology·Yeun-Kyung ShinYan Zhou
Sep 18, 2008·The Journal of General Virology·Benjamin G HaleDavid Jackson
Mar 18, 2009·Cellular Microbiology·Christina Ehrhardt, Stephan Ludwig
Jun 6, 2009·Journal of Virology·Jeffrey E McLeanZahra F Zakeri
Nov 3, 2009·Virology·David JacksonRichard E Randall
Feb 5, 2010·The Journal of General Virology·Xinya LuYan Zhou
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Benjamin G HaleRupert J Russell
Feb 11, 2010·Cell·Jan RehwinkelCaetano Reis e Sousa
Apr 2, 2010·The Journal of General Virology·Claudius SeitzUdo Reichl
Aug 10, 2010·Vaccine·B HeynischU Reichl
Dec 14, 2011·Journal of Virological Methods·Stephen M LindsayJohn Yin
Dec 22, 2011·The Lancet Infectious Diseases·Aeron C HurtUNKNOWN WHO Consultation on Pandemic Influenza A (H1N1) 2009 Virus Resistance to Antivirals
Aug 23, 2012·PloS One·Koji OnomotoTakashi Fujita
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 9, 2012·Journal of Virology·Anh T TranKevin M Coombs
Mar 26, 2013·Antiviral Research·Mélanie SamsonGuy Boivin
Feb 5, 2014·Trends in Biotechnology·Chengjun LiHualan Chen
Citations
Jan 15, 2016·Journal of Natural Medicines·Kohei SasakiToshimitsu Hayashi
Feb 2, 2021·Bioscience of Microbiota, Food and Health·Takumi WatanabeToshio Kawahara
May 19, 2021·PloS One·Takumi WatanabeToshio Kawahara