Characterization of a novel peptide with allatotropic activity in the fall armyworm Spodoptera frugiperda

Regulatory Peptides
Mohatmed Abdel-LatiefKlaus H Hoffmann

Abstract

A cDNA that encodes 53 amino acids, including one copy of the RVRGNPISCF-OH peptide, was cloned from Spodoptera frugiperda. This peptide strongly stimulates the synthesis and release of juvenile hormone (JH) in vitro by the corpora allata (CA) of S. frugiperda and was code-named Spofr-AT 2. Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses revealed that the preprohormone is expressed as one transcript in the brain, midgut (Mg) and ovary (Ov) in a tissue- and developmental-specific manner. Whole-mount in situ hybridization confirmed the gene expression in the suboesophageal ganglion (SOG) and in the ovary of adult females. Treating the CA with the synthetic peptide caused an up to tenfold increase in the release of JH. The stimulation was dose-dependent with an apparent EC(50) of ca. 10(-7) M. CA that were activated with Spofr-AT 2 could be inhibited by the addition of synthetic allatostatin type-C from Manduca sexta (Manse-AS). This is the first report on the presence and function of two different peptides with allatotropic activity in an insect species.

References

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S J KramerD A Schooley
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Aug 1, 1996·European Journal of Biochemistry·P A TaylorF M Horodyski
Feb 17, 2000·Annals of the New York Academy of Sciences·W G BendenaS S Tobe
Mar 27, 2001·Biochemical and Biophysical Research Communications·M WilliamsonC J Grimmelikhuijzen
May 31, 2001·Genome Research·R S Hewes, P H Taghert
May 9, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sebastian RangeKlaus H Hoffmann
May 29, 2003·Journal of Insect Physiology·M -P. TuJ G. Stoffolano
May 29, 2003·Journal of Insect Physiology·K J. Yagi, S S. Tobe
Mar 18, 2004·Archives of Insect Biochemistry and Physiology·Mohatmed Abdel-LatiefKlaus H Hoffmann
Mar 17, 1989·Science·H KataokaS J Kramer

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Citations

Sep 9, 2008·General and Comparative Endocrinology·N Audsley, R J Weaver
Apr 2, 2008·Journal of Insect Physiology·N AudsleyR J Weaver
May 22, 2009·Annals of the New York Academy of Sciences·R J Weaver, N Audsley
Jun 15, 2010·Archives of Insect Biochemistry and Physiology·Mohatmed Abdel-latief, Klaus H Hoffmann
Jan 27, 2015·Journal of Insect Physiology·Yu Kaneko, Kiyoshi Hiruma
May 24, 2014·Journal of Insect Physiology·Intisar T E HassanienKlaus H Hoffmann

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