Characterization of a novel phage infecting the pathogenic multidrug-resistant Bacillus cereus and functional analysis of its endolysin

Applied Microbiology and Biotechnology
Qin Peng, Yihui Yuan

Abstract

Bacillus cereus is widely distributed food-borne pathogenic bacterium. Due to the harmness to human hearth and the generation of multidrug-resistant B. cereus, it is urgent to develop novel antimicrobial agents. Phage and phage endolysin were taken as novel antimicrobial substance for their specific lytic activity against pathogenic bacteria. In this study, a Myoviridae family phage, designated as vB_BceM-HSE3, infecting the pathogenic multidrug-resistant B. cereus strain was isolated and characterized along with its endolysin. Phage vB_BceM-HSE3 can specially infect the B. cereus group strains, including B. cereus, B. anthracis, and B. thuringiensis, and exhibits high temperature and pH tolerance, which endow it with high potential for been used in controlling pathogenic B. cereus group strains. Genomic analysis reveals that vB_BceM-HSE3 is a novel phage and only shows extremely low genome similarity with available phage genome. Functional analysis of endolysin PlyHSE3 encoding by vB_BceM-HSE3 shows that PlyHSE3 exhibits broader lytic spectrum than the phage and can lyse all the tested B. cereus group strains as well as the tested pathogenic strain of P. aeruginosa. PlyHSE3 also shows broad temperature and pH tolerance, and ca...Continue Reading

References

Aug 1, 1991·Vaccine·P C Turnbull
Oct 1, 1993·Clinical Microbiology Reviews·F A Drobniewski
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Dec 26, 2001·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Sep 13, 2002·Current Topics in Microbiology and Immunology·T M Koehler
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Dec 19, 2003·Nucleic Acids Research·Naomi SiewDaniel Fischer
Oct 14, 2004·Bioinformatics·Paul Stothard, David S Wishart
Nov 9, 2004·Bioinformatics·Johannes Söding
Jun 7, 2005·Trends in Microbiology·Mya Breitbart, Forest Rohwer
Jun 28, 2005·Current Opinion in Microbiology·Martin J Loessner
Jun 28, 2005·Nucleic Acids Research·Peter SchattnerTodd M Lowe
Aug 17, 2005·The Journal of Biological Chemistry·Lieh Yoon LowRobert C Liddington
Aug 30, 2005·Trends in Microbiology·Vincent A Fischetti
Feb 21, 2007·Bioinformatics·Jan KrumsiekThomas Rattei
Aug 2, 2007·Canadian Journal of Microbiology·G T Vilas-BôasO M N Arantes
Nov 2, 2007·Science in China. Series C, Life Sciences·Yao WangShiYun Chen
Apr 22, 2008·FEMS Microbiology Reviews·Lotte P Stenfors ArnesenPer Einar Granum
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marco J MorelliRosalind J Allen
Mar 30, 2010·Nature Reviews. Microbiology·Simon J LabrieSylvain Moineau
Apr 9, 2010·Clinical Microbiology Reviews·Edward J Bottone
Jun 4, 2010·The Journal of Biological Chemistry·James E ThompsonIan S Roberts
Dec 1, 2011·Nucleic Acids Research·Marco PuntaRobert D Finn
Nov 29, 2013·The Journal of General Virology·Yihui YuanYan Wu
Mar 4, 2014·The Journal of Microbiology·Ryland Young
Jan 18, 2015·Applied and Environmental Microbiology·Minsuk Kong, Sangryeol Ryu
Jun 28, 2015·Journal of Biotechnology·Xiankai LiuHengliang Wang
Oct 27, 2015·Genome Announcements·Bryan D MerrillSandra H Burnett
Nov 19, 2015·Nucleic Acids Research·Lihong ChenQi Jin
Dec 29, 2015·Current Opinion in Biotechnology·Mathias Schmelcher, Martin J Loessner
May 18, 2016·FEMS Microbiology Letters·Hongjun NaSangryeol Ryu
Sep 24, 2016·Applied Microbiology and Biotechnology·Ganesh Ram R VisweswaranGirbe Buist
Nov 5, 2016·BMC Infectious Diseases·Breanne M HeadAdrienne F A Meyers
Nov 12, 2016·International Journal of Food Microbiology·Peiling GengXiaomin Hu
Feb 19, 2017·Food Microbiology·Alizée GuérinChristophe Nguyen-The

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Citations

Sep 25, 2019·Antibiotics·Steven M SwiftDaniel C Nelson
Dec 2, 2020·Microorganisms·Alice ChateauAntonella Fioravanti
Jan 6, 2021·Nucleic Acids Research·Delia Casas-PastorGeorg Fritz
Mar 20, 2021·World Journal of Microbiology & Biotechnology·Adriana Lopez-ArvizuIsrael García-Cano

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Datasets Mentioned

BETA
MF418016
M
2017798

Methods Mentioned

BETA
transmission electronic microscopy

Software Mentioned

Gepard
tRNAScan
GeneMarks
CGview
Pfam
HHpred
Velet

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