Characterization of a novel plasmid encoding F4-like fimbriae present in a Shiga-toxin producing enterotoxigenic Escherichia coli isolated during the investigation on a case of hemolytic-uremic syndrome

International Journal of Medical Microbiology : IJMM
Valeria MichelacciStefano Morabito

Abstract

In February 2017 a case of Hemolytic-Uremic Syndrome (HUS) was reported to the National Registry of HUS in an adult living in Northern Italy. Stool specimens from the patient and his family contacts were collected and the analyses led to the isolation of a Locus of Enterocyte Effacement (LEE)-negative Shiga toxin 2 (Stx2)-producing Escherichia coli. The epidemiological investigations performed brought to collect fecal samples from the animals reared in a farm held by the case's family and a mixture of bovine and swine feces proved positive for Shiga toxin-producing E. coli (STEC) and yielded the isolation of a LEE-negative stx2-positive E. coli strain. Further characterization by whole genome sequencing led to identify the isolates as two identical O2:H27 hybrid Enterotoxigenic Shiga toxin-producing E. coli (ETEC-STEC). Sequencing of a high molecular weight plasmid present in the human isolate disclosed a peculiar plasmid harboring virulence genes characteristic for both pathotypes, including the enterohemolysin-coding gene and sta1, encoding the heat stable enterotoxin. Moreover, a complete fae locus encoding the ETEC F4 fimbriae could be identified, including a novel variant of faeG gene responsible for the production of the ...Continue Reading

Citations

Aug 9, 2020·Frontiers in Cellular and Infection Microbiology·Ana Carolina de Mello SantosTânia Aparecida Tardelli Gomes
Aug 6, 2020·Frontiers in Microbiology·Michele ZuppiStefano Morabito
Feb 25, 2021·Emerging Infectious Diseases·Federica GigliucciValeria Michelacci
Jun 4, 2021·Transboundary and Emerging Diseases·Stefania LauziRosangela Tozzoli

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