Characterization of a novel RXR receptor in the salmon louse (Lepeophtheirus salmonis, Copepoda) regulating growth and female reproduction

BMC Genomics
Christiane EichnerFrank Nilsen

Abstract

Nuclear receptors have crucial roles in all metazoan animals as regulators of gene transcription. A wide range of studies have elucidated molecular and biological significance of nuclear receptors but there are still a large number of animals where the knowledge is very limited. In the present study we have identified an RXR type of nuclear receptor in the salmon louse (Lepeophtheirus salmonis) (i.e. LsRXR). RXR is one of the two partners of the Ecdysteroid receptor in arthropods, the receptor for the main molting hormone 20-hydroxyecdysone (E20) with a wide array of effects in arthropods. Five different LsRXR transcripts were identified by RACE showing large differences in domain structure. The largest isoforms contained complete DNA binding domain (DBD) and ligand binding domain (LBD), whereas some variants had incomplete or no DBD. LsRXR is transcribed in several tissues in the salmon louse including ovary, subcuticular tissue, intestine and glands. By using Q-PCR it is evident that the LsRXR mRNA levels vary throughout the L. salmonis life cycle. We also show that the truncated LsRXR transcript comprise about 50% in all examined samples. We used RNAi to knock-down the transcription in adult reproducing female lice. This res...Continue Reading

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Citations

Dec 23, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jakkapong KluebsoongnoenApinunt Udomkit
Mar 29, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ann M TarrantBenni Winding Hansen

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Datasets Mentioned

BETA
A-MEXP-2224
BAF85823

Methods Mentioned

BETA
Light microscopy
PCR
PCRs
feature Extraction

Software Mentioned

Express
MrBayes
MIAME
BLASTX
CAP3
Blast
BlastP
Agilent feature Extraction
Phred
SeqClean

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