Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters

FEMS Microbiology Letters
Emmanuele SeveriGavin H Thomas

Abstract

The function of sialic acids in the biology of bacterial pathogens is reflected by the diverse range of solute transporters that can recognize these sugar acids. Here, we use an Escherichia coliDeltananT strain to characterize the function of known and proposed bacterial sialic acid transporters. We discover that the STM1128 gene from Salmonella enterica serovar Typhimurium, which encodes a member of the sodium solute symporter family, is able to restore growth on sialic acid to the DeltananT strain and is able to transport [(14)C]-sialic acid. Using the DeltananT genetic background, we performed a direct in vivo comparison of the transport properties of the STM1128 protein with those of sialic acid transporters of the major facilitator superfamily and tripartite ATP-independent periplasmic families, E. coli NanT and Haemophilus influenzae SiaPQM, respectively. This revealed that both STM1128 and SiaPQM are sodium-dependent and, unlike SiaPQM, both STM1128 and NanT are reversible secondary carriers, demonstrating qualitative functional differences in the properties of sialic acid transporters used by bacteria that colonize humans.

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Citations

Feb 22, 2012·Applied and Environmental Microbiology·Jean-Bernard LubinE Fidelma Boyd
Dec 30, 2010·Infection and Immunity·Carolyn MarionSamantha J King
Sep 21, 2011·PLoS Genetics·Jason W HolderAnthony J Sinskey
Aug 29, 2012·FEMS Microbiology Letters·Nadine GruteserGavin H Thomas
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Jul 17, 2020·The Journal of Biological Chemistry·Andrew BellNathalie Juge
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Sep 10, 2015·Microbiology Spectrum·Brandy L Haines-MengesE Fidelma Boyd

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