Characterization of a novel standardized human three-dimensional skin wound healing model using non-sequential fractional ultrapulsed CO2 laser treatments

Lasers in Surgery and Medicine
Yvonne MarquardtJens M Baron

Abstract

At present, there is no standardized in vitro human skin model for wound healing. Therefore, our aim was to establish and characterize an in vitro/ex vivo three-dimensional (3D) wound healing model, which we employed to analyze the effects of dexpanthenol on wound healing and gene regulation. The novel human 3D skin wound healing model using scaffold and collagen 3D organotypic skin equivalents was irradiated with a non-sequential fractional ultrapulsed CO2 laser. These standardized injured full-thickness skin equivalents enable qRT-PCR, microarray, and histological studies analyzing the effect of topically or systemically applied compounds on skin wound healing. These human laser-irradiated skin models were found to be appropriate for in vitro wound healing analysis. Topical treatment of skin wounds with a 5% dexpanthenol water-in-oil emulsion or two different 5% dexpanthenol oil-in-water emulsions clearly enhanced wound closure compared to laser-irradiated untreated control models. To find out whether this positive effect is caused by the active substance dexpanthenol, laser-irradiated skin models were cultured in calciumpantothenate containing medium (20 μg/ml) compared to skin equivalents cultured without calciumpantothenat...Continue Reading

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Citations

May 16, 2017·The Journal of Dermatological Treatment·Ehrhardt ProkschStéphanie Boudon
Oct 26, 2018·Journal of the European Academy of Dermatology and Venereology : JEADV·K ClarysseJ Baron
Feb 7, 2019·Journal of Biomedical Materials Research. Part a·Franziska KreimendahlJens Malte Baron
Jun 25, 2019·Frontiers in Microbiology·Dora E Corzo-LeónDonna M MacCallum
Mar 26, 2021·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·F PahnkeP Elsner
Jan 20, 2020·Dermatology : International Journal for Clinical and Investigative Dermatology·Jens Malte BaronEhrhardt Proksch

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