Characterization of a Novel Strain of Bacillus thuringiensis.

Applied and Environmental Microbiology
J E López-Meza, Jorge E Ibarra

Abstract

Bacillus thuringiensis is a well-known species of entomopathogenic bacteria that is widely used as a biopesticide against many insect pests. Insecticidal proteins, coded for by genes located in plasmids, form typical parasporal, crystalline inclusions during sporulation. In this report, an unusual strain of B. thuringiensis subserovar oyamensis (LBIT-113), isolated from living larvae of Anopheles pseudopunctipennis in Mexico, was characterized by its ultrastructure, the protein composition of its parasporal crystal, plasmid pattern, and toxicological properties against several insect and noninsect targets. The parasporal crystal is enclosed within the spore's outermost envelope (exosporium), as determined by transmission electron microscopy, and exhibits a square, flat shape. Its main components are two proteins with sizes of 88 and 54 kDa. Despite some crystal morphology resemblance, both proteins are immunologically unrelated to the Cry IIIA protein, as shown by immunoblot analysis, when probed with antisera raised against the 88-kDa protein and the Cry IIIA protein. Partial N-terminal sequence of the 88-kDa protein revealed a unique amino acid arrangement among the Cry proteins. Solubilization of the crystal proteins was ach...Continue Reading

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Citations

Dec 18, 2013·World Journal of Microbiology & Biotechnology·Jenia A M TuftsShawn P Ryan
Sep 3, 2013·Indian Journal of Microbiology·Ketan D Patel, Sanjay S Ingle
Feb 9, 2010·Folia Microbiologica·N M Rosas-GarcíaJ M Villegas-Mendoza
May 1, 1999·Applied and Environmental Microbiology· del Rincon-Castro MCJ E Ibarra
Mar 15, 2011·Journal of Bacteriology·Yiguang ZhuMing Sun
Jul 27, 2010·Applied and Environmental Microbiology·Pedro A Noguera, Jorge E Ibarra
Dec 7, 2011·Journal of Basic Microbiology·Ketan D PatelSanjay S Ingle
Jan 10, 2019·3 Biotech·J Francisco Castillo-EsparzaJorge E Ibarra

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