Characterization of a novel thermostable GH7 endoglucanase from Chaetomium thermophilum capable of xylan hydrolysis

International Journal of Biological Macromolecules
Chengyao HuaLusheng Zhu

Abstract

A new endoglucanase encoding gene (ctendo7) was cloned from the thermophilic fungus Chaetomium thermophilum and heterologously expressed in Pichia pastoris. The recombinant CTendo7 enzyme was purified by Ni2+ affinity chromatography and subsequently characterized. CTendo7 belongs to glycoside hydrolase family 7, and exhibited considerable activity against sodium carboxymethyl cellulose (CMC-Na) and xylan of 1.91 IU/mg and 3.05 IU/mg at the optimum reaction condition of 55 °C, pH 5.0, respectively. The purified enzyme displayed relatively good thermostability. The residual endoglucanase and xylanase activities were 74.3% and 66.2% after a 60 min pre-incubation at 70 °C. Additionally, Ag+, Fe3+ and Cu2+ negatively affected the enzyme's activity, while the presence of 1 mM and 5 mM Mn2+ significantly enhanced both endoglucanase and xylanase activities. Generation of soluble oligosaccharides from lignocellulose is a critical step in bioethanol production, and it is noteworthy that CTendo7 produced cello-oligosaccharides and xylo-oligosaccharides from the continuous enzymatic saccharification of CMC-Na and xylan, respectively. This is the first detailed report on a novel bifunctional endoglucanase/xylanase enzyme from C. thermophilu...Continue Reading

References

Dec 1, 1994·Current Opinion in Structural Biology·J D McCarter, S G Withers
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Jun 13, 2002·Bioresource Technology·Ye Sun, Jiayang Cheng
Jan 17, 2007·Carbohydrate Research·Sophie BlanchardSylvain Cottaz
Feb 10, 2007·Science·Michael E HimmelThomas D Foust
Mar 16, 2007·Microbial Cell Factories·Pernilla TurnerEva Nordberg Karlsson
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·A Joe ShawLee R Lynd
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Dec 17, 2009·Bioresource Technology·E VlasenkoF Xu
Apr 2, 2011·Applied Microbiology and Biotechnology·Lei ChangHong Lu
Dec 22, 2011·Current Opinion in Biotechnology·Gregg T BeckhamMichael E Himmel
Sep 28, 2013·Biotechnology for Biofuels·Ragothaman M YennamalliTaner Z Sen
Dec 10, 2013·Biochimica Et Biophysica Acta·Teemu HaikarainenAnastassios C Papageorgiou
May 28, 2016·Genome Announcements·Sandra SmitWendy W J van de Sande
Jul 18, 2017·International Journal of Biological Macromolecules·Uma Shankar Prasad UdayBiswanath Bhunia
Nov 24, 2017·Biotechnology for Biofuels·Timo SchuergSteven W Singer

❮ Previous
Next ❯

Citations

May 10, 2019·International Journal of Molecular Sciences·Aline Vianna BernardiTaisa Magnani Dinamarco
Oct 20, 2019·Applied Microbiology and Biotechnology·Priyanka Bajaj, Ritu Mahajan
Nov 25, 2020·Applied Microbiology and Biotechnology·Benjarat BunterngsookVerawat Champreda
Feb 8, 2021·International Journal of Biological Macromolecules·Shohreh AriaeenejadGhasem Hosseini Salekdeh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Barrett Esophagus

Barrett’s esophagus if a serious complication of gastroesophageal reflux disease during which the normal esophageal lining changes to tissue that resembles intestinal lining. Here is the latest research.