Characterization of a novel thiol activated phospholipase TAPLB1 from Trichosporon asahii MSR 54

International Journal of Biological Macromolecules
Ashima DuaRani Gupta

Abstract

Phospholipases are hydrolytic enzymes that play crucial roles in vivo and also possess immense biotechnological potential. In the present study, the phospholipase B of Trichosporon asahii MSR54 was overexpressed in E. coli and characterized. The 68-kDa enzyme was monomeric in solution and possessed phospholipase, lysophospholipase, esterase and acyltransferase activities. It was maximally active at pH 8.0 and 40 °C. The enzyme retained >50% activity between pH 3.0-8.0 and had a half-life of 30 min at 60 °C. Its activity was not metal dependent and was stable in the presence of most metal ions. Its catalytic efficiency on lysophosphatidyl choline was 1.0 × 103 mM-1 h-1. Site directed mutagenesis revealed R121 (present in the GYRAMV motif), S194 (present in the conserved GLSGG motif) and D420 (present in LVDXGE motif) to be the crucial amino acid residues for esterolytic activity. S194 and D420 were also the catalytic amino acids for lysophospholipase and phospholipase activities of the enzymes, while R121 was not involved in catalysis of phospholipid substrates. Further, it was found that cysteine residues in C61 and C354 were involved in disulphide linkages that imparted the properties of thiol activation and thermostability, r...Continue Reading

References

Jul 1, 1996·Bioscience, Biotechnology, and Biochemistry·H OishiY Tamai
Jan 11, 2000·Clinical Microbiology Reviews·M A Ghannoum
Oct 9, 2003·Journal of Medical Microbiology·Annemarie Borst, Ad C Fluit
Jun 24, 2005·Biotechnology Letters·Renate Ulbrich-HofmannLydia Bezakova
Nov 3, 2005·Mycoses·Martin SchallerBernhard Hube
Feb 24, 2006·Bioscience, Biotechnology, and Biochemistry·Shuji FujinoYouichi Tamai
Jan 16, 2007·Applied Microbiology and Biotechnology·L De MariaS Patkar
Sep 1, 2009·Journal of Water and Health·Arturo Casadevall, Liise-Anne Pirofski
Feb 23, 2011·International Journal of Molecular Sciences·Gregory S Richmond, Terry K Smith
Sep 5, 2015·International Journal of Molecular Sciences·Grazia M Borrelli, Daniela Trono

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