PMID: 9188610Jul 1, 1997Paper

Characterization of a novel third member of the human cytomegalovirus glycoprotein H-glycoprotein L complex

Journal of Virology
M T Huber, Teresa Compton

Abstract

A prerequisite for understanding the molecular function of the human cytomegalovirus (HCMV) gH (UL75)-gL (UL115) complex is a detailed knowledge of the structure of this complex in its functional form, as it is present in mature virions. The gH protein is known to be a component of a 240-kDa envelope complex designated as gCIII (D. R. Gretch, B. Kari, L. Rasmussen, R. C. Gehrz, and M. F. Stinski, J. Virol. 62:875-881, 1988). However, the exact composition of the gCIII complex remains unknown. In this report, we attempted reconstitution of the gCIII complex by coexpression of gH and gL in the baculovirus expression system. Formation of recombinant gH-gL complexes of approximately 115 kDa was demonstrated; however, no higher-molecular-mass (approximately 240-kDa) recombinant gH-gL complexes were detected, suggesting that the presence of gH and gL alone is not sufficient for reconstitution of the gCIII complex. To identify other mammalian and/or HCMV factors which may be necessary for gCIII formation, immunoprecipitates of gH and gL from HCMV-infected fibroblasts and purified HCMV virions were examined. This analysis did reveal a number of coprecipitating proteins which associate either transiently or integrally with gH and gL. On...Continue Reading

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Citations

Feb 12, 2002·Journal of Virology·Yingguang Liu, Bonita J Biegalke
May 22, 2002·Journal of Virology·Matthew Lopper, Teresa Compton
Nov 16, 2012·Human Vaccines & Immunotherapeutics·Devon J ShedlockDavid B Weiner
Nov 13, 2015·Journal of Virology·Gang Li, Jeremy P Kamil
Jun 17, 2016·Microbiology and Molecular Biology Reviews : MMBR·Thomas J Gardner, Domenico Tortorella
Mar 29, 2003·Journal of Virology·Teresa ComptonRobert W Finberg
Dec 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dai Wang, Thomas Shenk
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karl W BoehmeTeresa Compton
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Gang LiJeremy P Kamil
Apr 7, 2016·The Journal of General Virology·Kerstin Laib SampaioChristian Sinzger
May 28, 2005·Journal of Virology·Eric R Kinzler, Teresa Compton
Jan 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Claudio CiferriAndrea Carfi
Jan 7, 1998·Journal of Virology·A JönsT C Mettenleiter
Dec 14, 2018·Viruses·Christopher C Nguyen, Jeremy P Kamil

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