Characterization of a novel tyrosine phosphorylated 100-kDa protein that binds to SHP-2 and phosphatidylinositol 3'-kinase in myeloid cells.

The Journal of Biological Chemistry
K Carlberg, Larry R Rohrschneider

Abstract

Fms is a tyrosine kinase-containing receptor for macrophage colony-stimulating factor (M-CSF) that regulates survival, growth, and differentiation of cells along the monocyte/macrophage lineage. M-CSF stimulation of murine myeloid FDC-P1 cells expressing Fms resulted in the tyrosine phosphorylation of a number of signal transduction proteins, including an unidentified 100-kDa protein. This 100-kDa protein associated with the tyrosine phosphatase SHP-2 but not with the related phosphatase SHP-1. The kinetics of tyrosine phosphorylation of p100 and SHP-2 suggest that p100 may be a direct substrate of SHP-2. p100 bound directly to the SH2 domains of both SHP-2 and the p85 subunit of phosphatidylinositol 3'-kinase. The 100-kDa protein did not appear to bind directly to Fms, Ship, Cbl, Shc, or Grb2, although all of these proteins were coimmunoprecipitated with p85 after M-CSF stimulation. Association of p100 with SHP-2 and p85 did not require the major autophosphorylation sites on Fms nor binding of p85 to Fms. A tyrosine phosphorylated protein of 100 kDa also coprecipitated with SHP-2 from several other myeloid cell lines after M-CSF stimulation but was not seen in immunoprecipitates from Rat2 fibroblasts expressing Fms. Stimulatio...Continue Reading

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Citations

Sep 23, 1997·Biochemical and Biophysical Research Communications·S H OngG R Guy
Jun 1, 1997·Current Opinion in Immunology·B G Neel
Oct 1, 2003·Molecular and Cellular Biology·Albéna PramatarovaBrian W Howell
Oct 16, 2003·Molecular and Cellular Biology·Yehenew M Agazie, Michael J Hayman
Mar 18, 2003·Molecular and Cellular Biology·Martina SeiffertLarry R Rohrschneider
Jan 5, 2012·Advances in Hematology·Sheetal VermaKevin D Bunting
Mar 8, 2000·Growth Factors·R P Bourette, L R Rohrschneider
Jun 23, 2015·Molecular Medicine Reports·Chen-Bo DingJun-Min Luo
Mar 31, 2007·Biochemical and Biophysical Research Communications·Jae Cheal Yoo, Michael J Hayman
Nov 14, 2006·Biochemical and Biophysical Research Communications·Jae Cheal Yoo, Michael J Hayman
Jun 25, 2004·Molecular & Cellular Proteomics : MCP·Kevin WilhelmsenPeter van der Geer
May 29, 2002·Oncogene·Blanca Scheijen, James D Griffin
Apr 12, 2002·FEBS Letters·Yan Liu, Larry R Rohrschneider

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